皂荚素对大鼠DNA损伤的干预及对血清胆固醇的影响

为了探究皂荚素对高胆固醇模型大鼠抗氧化能力和DNA损伤以及对大鼠血浆胆固醇及相关血脂生化指标的影响,将48只健康大鼠，随机分为对照组、低浓度皂荚素组和高浓度皂荚素组，各组分别喂饲皂荚素质量比分别为0，80，200 mg/kg的饲料，制作由不同浓度H₂O₂(0，6，12，18 μmol/L)诱导的DNA损伤兼高胆固醇大鼠模型.实验期限为60 d，实验结束采血，应用试剂盒检测血清丙二醛(MDA)水平和谷胱甘肽过氧化物酶(GSH-PX)活性，并采用单细胞凝胶电泳法测定淋巴细胞DNA的损伤情况.结果显示，与对照组比较，低浓度皂荚素组、高浓度皂荚素组MDA含量和血清GSH活性差异均具有统计学意义；低浓度皂荚素组和高浓度皂荚素组大鼠血清总胆固醇TC、甘油三酯(TG)、载脂蛋白B(apoB)的含量都有明显的降低，尤其以高浓度皂荚素组最为显著;两组大鼠的胆汁酸排泄量增加，载脂蛋白A-Ⅰ(apoA-Ⅰ)、高密度脂蛋白-胆固醇(HDL-C)水平影响差异无统计学意义.说明皂荚素可有效降低血浆胆固醇水平，对于健康幼年大鼠血清GSH及脂质过氧化产物MDA含量有显著影响，对H₂O₂诱导的DNA损伤也表现出明显的保护作用.

Effects of Gymnocladas Saponin DNA Damage and Serum Cholesterol in Rats

To investigate the effects of gymnocladas saponin's antioxidant and DNA damage intervention capacity on rats with high cholesterol levels as well as the effects of gymnocladas saponin on serum cholesterol and relative serum biochemical indexes on targets,48 healthy rats were randomly divided into three groups:a control group,a low concentration gymnocladas saponin group and a high concentration gymnocladas saponin group.Rats of three groups respectively fed with 0，80，200 mg/kg gymnocladas saponin,who then have DNA damage and high cholesterol levels are induced byH₂O₂ (0,6,12,18 μmol/L) of different concentration.After 60 days,the collection of the blood sample obtained with the kits are used to test serum level and activity of malondialdehyde (MDA) and glutathione peroxidase (GSH-PX) as well as the damage of lymphocyte DNA by single cell gel electrophoresis (SCGE).As a result of the test,compared with the control group,the different content of MDA and the different activity of GSH in the low concentration gymnocladas saponin group and the high concentration gymnocladas saponin group have statistical significance.The low concentration of gymnocladas saponin group and the high concentration of gymnocladas saponin groups' serum total cholesterol (TC),triglyceride (TG),apolipoprotein B (apoB) are significantly reduced,especially in the high concentration of gymnocladas saponin group.At the same time,the bile acid excretion in two groups of rats increases.The diffenrence of Apolipoprotein A-Ⅰ (apoA-Ⅰ) and high density lipoprotein cholesterol (HDL-C) level effect has no statistical significance.This illustrates that gymnocladas saponin can effectively reduce the level of plasma cholesterol,and can have significant effect on the content of GSH and MDA in serum of healthy young rats and on the protection of DNA damage induced byH₂O₂.