| 磁场影响固定化葡萄糖异构酶活性的研究 |
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| 引用本文： | 贺华君,朱元保.磁场影响固定化葡萄糖异构酶活性的研究[J].吉首大学学报(自然科学版),1999,20(1):20-24. |
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| 作者姓名： | 贺华君 朱元保 |
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| 作者单位： | [1]湖南大学化学化工学院 [2]华东理工大学生物化工学院 |
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| 基金项目： | 国家自然科学基金资助课题 |
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| 摘 要： | 采用丹麦Novo公司测固定化葡萄糖异构酶 (SweetzymeT)活力的反应体系 ,用半胱氨酸 -咔唑法评价酶的活性 ,研究了磁场在不同条件下对酶活力的影响。实验发现 :在不同磁化时间、温度、磁场强度和pH条件下磁化底物 ,使其活性增加可达 2 8 6 % ,并且具有可逆性 ,但磁化固定化酶或磁场作用于酶—底物反应体系 ,未见酶活力的明显变化。
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| 关 键 词： | 磁场 固定化葡萄糖异构酶 酶活性 果糖 |
The Effect of Magnetic Field on Immobilized Glucose Isomerase Catalytic Activity |
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| He Huajun,Zhu Yuanbao,Cao Zhixiang,He Shuang-e,Liang Huicong.The Effect of Magnetic Field on Immobilized Glucose Isomerase Catalytic Activity[J].Journal of Jishou University(Natural Science Edition),1999,20(1):20-24. |
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| Authors: | He Huajun Zhu Yuanbao Cao Zhixiang He Shuang-e Liang Huicong |
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| Institution: | He Huajun 1Zhu Yuanbao 1Cao Zhixiang 1He Shuang-e 3Liang Huicong 1 |
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| Abstract: | By Novo method for activity determination of the Immobilized Glucose Isomerase (IGI)-Sweetzyme T,the effect of magnetized substrate on IGI activity was studied. It was found the activity has been enhanced 28.6% when the substrate was under 125mT magnetization for 4h,the effect is reversible. However, when the magnetic field was imposed on IGI or IGI-substrate reaction system, no significant change was observed. |
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| Keywords: | magnetic field immobilized glucose isomerase enzyme activity fructose |
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