| 一株耐高温纤维素降解菌的分离筛选及鉴定 |
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| 引用本文： | 易旻,杨玉婷,李梦霖,张丽,贺建武,刘祝祥,陈义光.一株耐高温纤维素降解菌的分离筛选及鉴定[J].吉首大学学报(自然科学版),2017,38(4):72-77. |
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| 作者姓名： | 易旻 杨玉婷 李梦霖 张丽 贺建武 刘祝祥 陈义光 |
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| 作者单位： | （1.吉首大学生物资源与环境科学学院，湖南 吉首 416000;2.杜仲综合利用技术国家地方联合工程实验室，湖南吉首 416000;3.(武陵山区)植物资源保护与利用湖南省高校重点实验室，湖南 吉首 416000) |
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| 基金项目： | 国家自然科学基金资助项目（31460004）;湖南省重点学科建设项目（JSU071312Z01）;吉首大学校级科研项目（JDY16010） |
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| 摘 要： | 为了分离筛选耐高温的纤维素高效降解菌，从鸡粪蘑菇渣高温堆肥中取样，采用滤纸富集培养基进行富集培养、刚果红纤维素培养基进行分离纯化培养、滤纸条培养基和刚果红纤维素培养基进行初筛培养、液体发酵测定纤维素酶活法进行复筛培养，利用形态特征观察、生理生化特征检测和基于16S rRNA基因序列的系统发育分析法进行初步鉴定，结果分离筛选到1株耐高温纤维素降解菌XD-3.该菌株在50 ℃生长良好、羧甲基纤维素酶(CMCase)活力达6.14±0.37 U/mL.综合形态特征、生理生化特性、基于16S rRNA基因序列的系统发育分析，菌株XD-3初步鉴定为解淀粉芽孢杆菌（Bacillus amyloliquefaciens）.
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| 关 键 词： | 耐高温纤维素降解菌 16S rRNA基因 系统发育分析 解淀粉芽孢杆菌 |
Screening and Identification of a High-Temperature Resistant Cellulose-Degrading Bacterium |
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| YI Min,YANG Yuting,LI Menglin,ZHANG Li,HE Jianwu,LIU Zhuxiang,CHEN Yiguang.Screening and Identification of a High-Temperature Resistant Cellulose-Degrading Bacterium[J].Journal of Jishou University(Natural Science Edition),2017,38(4):72-77. |
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| Authors: | YI Min YANG Yuting LI Menglin ZHANG Li HE Jianwu LIU Zhuxiang CHEN Yiguang |
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| Institution: | （1.College of Biology and Environmental Sciences，Jishou University，Jishou 416000，Hunan China;2．National & Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia Ulmoides，Jishou 416000，Hunan China;3.Key Laboratory of Plant Resources Conservation and Utilization (College of Hunan Province)，Jishou University，Jishou 416000，Hunan China) |
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| Abstract: | In order to obtain high-temperature resistant cellulose-degrading bacteria，samples were collected from chicken manure and mushroom residue compost.After being enriched through filter paper medium，high-temperature resistant cellulose-degrading bacteria were isolated and purified on cellulose Congo red medium.The isolates were preliminarily screened through filter medium and cellulose Congo red medium，and then were further screened through quantitative analysis of cellulase activity of liquid fermentation broth.A high-temperature resistant cellulose-degrading bacterium，designated strain XD-3，was obtained，which grew well at 50 ℃ and the carboxymethyl-cellulase (CMCase) activity was 6.14±0.37 U/mL.The combination of morphology，physiological/biochemical characterization and phylogenetic analysis based on 16S rRNA gene sequence showed that strain XD-3 was a member of a known Bacillus species，Bacillus amyloliquefaciens. |
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| Keywords: | high-temperature resistant cellulose-degrading bacteri 16S rRNA gene phylogenetic analysis Bacillus amyloliquefaciens |
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