18-碳酸-3,4-呋喃二酯对小鼠精子超微结构的影响

研究了18-碳酸-3,4-呋喃二酯对昆明小鼠精子超微结构的影响.处死10只健康有生育力的雄性小鼠,取睾丸组织,获取具有高活力的精子悬浮液,测定18-碳酸-3,4-呋喃二酯的瞬间杀精活性；然后将经过处理的精子团块固定在2.5%的戊二醛溶液中,电镜观察.结果表明，18-碳酸-3,4-呋喃二酯的瞬间杀精浓度为4 g·L^-1.在电镜下,精子头部顶体与核膜被破坏,在核质膜周边形成大小不一的虫蚀状缺痕,严重者,细胞核膜破裂/缺损,核染色质不均匀,呈颗粒样改变,有空泡形成.由此可见,18-碳酸-3,4-呋喃二酯有很强的体外杀精作用.

Ultrastructural Effect of Octadecanoic Acid-3,4-Furanodiyl Ester on Sperm in Male Mice

The effects of octadecanoic acid-3,4-furanodiyl ester on Kunming male mice sperm ultra-structural characteristics were studied in vitro. Kill 10 healthy fertile mice and collect the testicle, and, with a swim-up technique, a sperm suspension of high motility and purity was extract of neem oil,and the sperm pellets were fixed in 2.5% glutaraldehyde (pH was 7.2) and observed in electron microscope. Octadecanoic acid-3,4-furanodiyl ester can kill all sperm of Kunming mice in vitro in less than 20 s at the concentration of 4 g/L. On electron microscope, the acrosome and karyotheca of spermatids had been damaged. Small-to-large damaged areas which seemed to have been bitten by worms could be observed at the edge of the cytoplasmic membrane. In some cases, membrane and the peripheral mitochondria were virtually absent and the entire cytoplasm was medullary degenerated and vacuolated. The octadecanoic acid-3,4-furanodiyl ester has strong sperm-killing activity in vitro. There are evident histological changes due to octadecanoic acid-3,4-furanodiyl ester in sperm of male Kunming mice.