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磁场对脲酶影响
引用本文:钟科军,叶忆德.磁场对脲酶影响[J].吉首大学学报(自然科学版),1997,18(2):15-19.
作者姓名:钟科军  叶忆德
作者单位:湖南大学化学化工学院!长沙,410082,湖南大学化学化工学院!长沙,410082,湖南大学化学化工学院!长沙,410082,湖南大学化学化工学院!长沙,410082
摘    要:用电导法分别研究了磁处理水、酶液和反应体系对服酶酶促反应的影响。短时间磁处理反应体系未观察到其发生明显变化,分别处理水或酶溶液,均能降低酶促反应速度,其米氏常数和最大反应速度也相应减小,影响程度与磁场强度、磁化时间和温度条件有关.磁场强度大,磁处理时间长,活性加速降低程度越大.温度较低时其降低程度大,温度较高时,其影响开始逐渐减弱。

关 键 词:磁场  脲酶  磁化水

Effect of Magnetic Field on Urease
Zhong Kejun, Xie Yide, He Shuange, Zhu Yuanbao.Effect of Magnetic Field on Urease[J].Journal of Jishou University(Natural Science Edition),1997,18(2):15-19.
Authors:Zhong Kejun  Xie Yide  He Shuange  Zhu Yuanbao
Abstract:The influence of Magnetic field on Urease Cata-lyzed reaction was studied bymagnetize the water for substrate solution,the Urease solution and the reaction system. Theactivity of urease was measured by conductametric method. No significient change wasobserved for periods of exposure of the substrate-enzyme reaction system in magnetic field,Magnetize water or enzyme solution, however, the rate of reaction in high substrateconsentration and increase in low substrate concentraton,The Michaelis constent km and themaximum reaction rate Vmax also deerease correspondingly,the degree of magnetic effect arerelated to magnetization time. field intensity and temperature. High field-intensity,longmagnetization time and low temperature lead to significient change in the enzyme activitycompare to control.
Keywords:magnetic field  urease  magnetized water
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