| 应用响应面法优化铆钉菇菌丝体胞外多糖提取工艺 |
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| 引用本文： | 江洁,刘凯,刘丹华.应用响应面法优化铆钉菇菌丝体胞外多糖提取工艺[J].大连民族学院学报,2011,13(3):241-244. |
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| 作者姓名： | 江洁 刘凯 刘丹华 |
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| 作者单位： | 大连民族学院生命科学学院,辽宁大连,116605 |
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| 基金项目： | 辽宁省教育厅项目，中央高校基本科研业务专项资金资助项目，大连民族学院太阳鸟项目 |
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| 摘 要： | 采用响应面法对铆钉菇菌丝体胞外多糖的提取工艺进行优化,以提取液中多糖含量为响应值。结果表明,影响铆钉菇菌丝体胞外多糖提取的主要因素依次为:提取温度、料液比、提取时间;获得的各因素最佳水平为:提取温度6℃,提取时间5 h,料液体积比1∶4.4。在此优化条件下,所得胞外多糖的质量浓度为278.86 mg.L-1,比优化前提高了20.40%。
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| 关 键 词： | 铆钉菇 菌丝体 胞外多糖 提取 响应面法 |
Optimization of Extraction Conditions of Exopolysaccharides from Gomphidius Rutilus Gomphidius Rutilus Using Response Surface Methodology |
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| GANG Jie,LIU Kai,LIU Dan-hua.Optimization of Extraction Conditions of Exopolysaccharides from Gomphidius Rutilus Gomphidius Rutilus Using Response Surface Methodology[J].Journal of Dalian Nationalities University,2011,13(3):241-244. |
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| Authors: | GANG Jie LIU Kai LIU Dan-hua |
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| Institution: | (College of Life Sciences,Dalian Nationalities University,Dalian Liaoning 116605,China) |
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| Abstract: | The extraction motheds for Gomphidius rutilus mycelium exopolysaccharides were optimized by response surface methodology（RSM）.The results showed that the degree of the factors significantly influencing the yield of polysaccharides was in order of extraction temperature,ratio of liquid to solid,extraction times.The optimum extraction conditions are as follows： the temperature 6 ℃,extraction time 5 hours and ratio of material to liquid 1：4.4.Under these optimum conditions,the exopolysaccharide obtained was 278.86mg/L,improved 20.40 % compared with that before optimization. |
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| Keywords: | gomphidius rutilus mycelium exopolysaccharides extraction response surface methodology |
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