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| PCR-DHPLC技术快速检测食品中英诺克李斯特氏菌 | |
| 引用本文： | 曹际娟,王耀,王顺芝,李天顺,李建民,赤列加措.PCR-DHPLC技术快速检测食品中英诺克李斯特氏菌[J].辽宁师范大学学报(自然科学版),2009,32(2):235-238. |
| 作者姓名： | 曹际娟 王耀 王顺芝 李天顺 李建民 赤列加措 |
| 作者单位： | 1. 辽宁出入境检验检疫局,辽宁,大连,116001 2. 辽宁出入境检验检疫局,辽宁,大连,116001;沈阳农业大学,植保学院,辽宁,沈阳,110161 3. 西藏出入境检验检疫局,西藏,拉萨,850002 |
| 摘 要： | 应用PCR结合变性高效液相色谱（DHPLC）技术，建立食品中英诺克李斯特氏菌的快速检测方法．根据英诺克李斯特氏菌的特有基因设计特异性引物，PCR扩增的产物经DHPLC技术进行快速检测，并以41株参考菌株做特异性试验．试验结果表明该方法具有很好的特异性，经PCR—DHPLC可检测食品中英诺克李斯特氏菌．该方法可以快速、准确检测英诺克李斯特氏菌，是食品微生物快速检测的新技术． |
| 关 键 词： | PCR—DHPLC 食品 英诺克李斯特氏菌 |
Identification of Listeria innocua in food by PCR-DHPLC |
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| CAO Ji-juan,WANG Yao,WANG Shun-zhi,LI Tian-shun,LI Jian-min,CHILIE Jia-cuo.Identification of Listeria innocua in food by PCR-DHPLC[J].Journal of Liaoning Normal University(Natural Science Edition),2009,32(2):235-238. | |
| Authors: | CAO Ji-juan WANG Yao WANG Shun-zhi LI Tian-shun LI Jian-min CHILIE Jia-cuo |
| Institution: | CAO Ji-juan , WANG Yao, WANG Shun-zhi , LI Tian-shun , LI Jian-min , CHILIE Jia-cuo (1. Liaoning Entry-Exit Inspection ＆ Quarantine Bureau, Dalian 116001,China; 2. Plant Protection College, Shenyang Agricultural University, Shenyang 110161 ,China; 3. Xizang Entry-Exit Inspection ＆Quarantine Bureau, Lasa 850002,China) |
| Abstract: | To identify Listeria innocua in food, a PCR-Denaturing High-Performance Liquid Chromatography （DHPLC） assay was performed in this study. Specific primers for specific gene of Listeria innocua were selected to conduct the PCR-DHPLC assays. The specific testing was performed with 41 strains. The good specificity of this method was found in the study. Also, it can detect and identify Listeria innocua quickly and accurately by a PCR-DHPLC. The DHPLC could be a new method for detecting pathogens in food. |
| Keywords: | PCR-DHPLC |
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