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转BADH基因玉米的获得及其耐盐性
引用本文:付光明,苏乔,吴畏,赵洪梅,安利佳.转BADH基因玉米的获得及其耐盐性[J].辽宁师范大学学报(自然科学版),2006,29(3):344-347.
作者姓名:付光明  苏乔  吴畏  赵洪梅  安利佳
作者单位:1. 武汉生物工程学院,生物技术系,湖北,武汉,430415
2. 大连理工大学,环境与生命学院,辽宁,大连,116024
3. 辽宁师范大学,生命科学学院,辽宁,大连,116029
基金项目:辽宁省科技基金资助项目(2001101001)
摘    要:利用花粉管通道技术将甜菜碱醛脱氢酶(BADH)基因导入玉米,以提高玉米的耐盐性.对1286株转化玉米进行PCR检测,共有16株呈阳性,转化率为1.2%.Southern Blot进一步证明BADH基因已经整合到玉米基因组.将阳性植株的后代(T3)用含1.2%NaCl的Hogland营养液每日1次浇灌。14d后对玉米叶片的相对电导率和叶绿素含量进行测定.结果表明在同样的盐胁迫条件下.转基因植株所受到的盐伤害明显较对照植株轻,说明转入的BADH基因可以提高玉米的耐盐能力.

关 键 词:花粉管通道  耐盐性  玉米  遗传转化  BADH
文章编号:1000-1735(2006)03-0344-04
收稿时间:03 24 2006 12:00AM
修稿时间:2006年3月24日

Transconduct BADH Gene into Maize and the Salt Tolerance of Transgenic Maize
FU Guang-ming,SU Qiao,WU Wei,ZHAO Hong-mei,AN Li-jia.Transconduct BADH Gene into Maize and the Salt Tolerance of Transgenic Maize[J].Journal of Liaoning Normal University(Natural Science Edition),2006,29(3):344-347.
Authors:FU Guang-ming  SU Qiao  WU Wei  ZHAO Hong-mei  AN Li-jia
Institution:1. Biotechnoiogy Department of Wuhan Bioengineering Institute, Wuhan 430415,China; 2. Environment and Life School of Dalian University of Technology, Dalian 116023 ,China; 3. College of Life Science, Liaoning Normal University, Dalian 116029, China
Abstract:Betaine aldehyde dehydrogenase(BADH) gene was transferred into Maize by using pollen-tube pathway method in order to enhance the salt tolerance of Maize.Investigation by PCR,shows that 16 of 1 286 transformed maizes are positive and the efficiency of transformation is 1.2%.Southern Blot indicated that BADH gene had been integrated into the genome of Maize.T_3 of positive Maize was planted in Hogland culture medium with 1.2% NaCl.The relative conductivity and chlorophyll content of the Maize was analyzed after growing for 14 days.The result of the experiment proved that the damage of salt to transgenic Maize was lower than the control under the same concentration treatment of salt,and it showed that the transformed BADH gene enhanced the salt tolerance of Maize.
Keywords:pollen-tube pathway  salt-tolerance  Maize genetic transformation  BADH
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