| 乳酸阈强度运动对糖尿病大鼠骨骼肌IRS-2蛋白含量及磷酸化的影响 |
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| 引用本文： | 王耀光,郭仁勇.乳酸阈强度运动对糖尿病大鼠骨骼肌IRS-2蛋白含量及磷酸化的影响[J].辽宁师范大学学报(自然科学版),2010,33(3). |
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| 作者姓名： | 王耀光 郭仁勇 |
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| 作者单位： | 1. 辽宁师范大学体育学院,辽宁,大连,116029
2. 辽宁师范大学生命科学学院,辽宁,大连,116029 |
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| 基金项目： | 辽宁省教育厅自然科学基金项目 |
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| 摘 要： | 观察乳酸阈强度运动对糖尿病大鼠骨骼肌胰岛素受体底物-2(IRS-2)蛋白含量及磷酸化的影响.在制备糖尿病大鼠模型后将SD大鼠分为3组:正常对照组(n=10),糖尿病对照组(n=10)和糖尿病运动组(20m/min组(n=10)).运动组每天运动50min,共6周.用Westernblot免疫印迹法检测骨骼肌细胞IRS-2蛋白磷酸化程度.结果显示:与糖尿病对照组相比,糖尿病运动组IRS-2蛋白含量升高24.4%(P0.01)、磷酸化提高37.2%(P0.01).本实验提示,乳酸阈强度运动对IRS-2蛋白含量和磷酸化程度产生明显影响.
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| 关 键 词： | 乳酸阈强度运动 糖尿病大鼠 骨骼肌 |
Effects of LT exercise onthe protein content and tyrosine phosphorylation of IRS-2in skeletal muscle of DM rats |
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| WANG Yao-guang,GUO Ren-yong.Effects of LT exercise onthe protein content and tyrosine phosphorylation of IRS-2in skeletal muscle of DM rats[J].Journal of Liaoning Normal University(Natural Science Edition),2010,33(3). |
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| Authors: | WANG Yao-guang GUO Ren-yong |
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| Institution: | WANG Yao-guang1,GUO Ren-yong2(1.Physical Education Institute of Liaoning Normal University,Dalian 116029,China,2.Life Science Institute of Liaoning Normal University,China) |
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| Abstract: | In this paper,we will explore effects of LT exercise on the protein content and tyrosine phosphorylation of IRS-2 in rats with diabetes.Thirty SD rats were divided into three groups:normal control group(NC,n=10),diabetes mellitus group(DMC,n=10)and exercise group(EXE,n=10)after DM model setting.The exercise groups did the running exercise each day for 50min with the intensity of 20m/min,lasting for six weeks.The content and tyrosine phosphorylation of IRS-2 protein were measured by Western bolt analysis.The... |
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| Keywords: | IRS-2 |
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