| 对3种不同硫化物自组膜免疫传感器的比较研究 |
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| 引用本文： | 孙越,刘媛媛,冯春梁.对3种不同硫化物自组膜免疫传感器的比较研究[J].辽宁师范大学学报(自然科学版),2007,30(2):207-209. |
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| 作者姓名： | 孙越 刘媛媛 冯春梁 |
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| 作者单位： | 辽宁师范大学化学化工学院,辽宁大连116029 |
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| 基金项目： | 国家自然科学基金资助项目（60572009）;辽宁省自然科学基金资助项目（210144）. |
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| 摘 要： | 在半胱氨酸自组膜上固定抗体（羊抗小鼠IgG）制备免疫传感器，分别与巯基十一酸自组膜和32-巯基-3，6，9，12，15，18，21-七氧杂三十二烷酸（EG6COOH）自组膜免疫传感器比较其性能。结果发现通过半胱氨酸自组膜制备的免疫传感器具有最好的灵敏度。能够在13min内。0．05～5．4μg／L范围对游离IgG灵敏检测，检测下限为0．05μg／L。
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| 关 键 词： | 免疫传感器 自组装单分子膜（SAM） 羊抗小鼠IgG抗体 |
| 文章编号： | 1000-1735（2007）02-0207-03 |
| 修稿时间： | 2006-09-02 |
A comparative study of immunosensors based on self-assembled monolayers formed from three different sulfides |
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| SUN Yue,LIU Yuan-yuan,FENG Chun-liang.A comparative study of immunosensors based on self-assembled monolayers formed from three different sulfides[J].Journal of Liaoning Normal University(Natural Science Edition),2007,30(2):207-209. |
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| Authors: | SUN Yue LIU Yuan-yuan FENG Chun-liang |
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| Institution: | College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China |
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| Abstract: | A procedure for the covalent coupling of goat anti-mouse IgG antibody(anti-IgG) to a gold surface modified with self-assembled monolayer (SAM) of cysteine was investigated.The performance of the SAM-antibody layer was compared to those of similar layers based on 11-mercaptoundecanoic acid(MUA) and 32-mercapto-3,6,9,12,15,18,21-heptaoxadotriacontan-1-oic acid(EG6COOH) by using label-free immunosensor system.The immunosensor modified with SAM of cysteine gave the highest sensitivity.It could detect the IgG in a range of 0.05～5.4 μg/L within 13 min,and the detection limit was 0.05 μg/L. |
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| Keywords: | immunosensor self-assembled monolayer(SAM) anti-IgG |
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