大叶饲料槐高频再生体系建立及试管苗工厂化生产

通过叶片外植体愈伤组织诱导和直接不定芽再生途径，建立了大叶饲料槐的高频再生系统，采用正交试验筛选出愈伤组织最适诱导培养基为；MS附加0．5mg／L 6-BA和1．0mg／L NAA；愈伤组织分化最适培养基为：MS附加2．0mg／L 6-BA和0．2mg／L NAA。叶片外植体直接分化最适培养基为：MS附加2．0mg／L 6-BA和0．1mg／L NAA；试管苗在1／2MS附加0．2mg／L IBA、0．1mg／L NAA和2g／L AC的培养基上生根率高达100％，移栽成活率达90％，从而实现了大叶饲料槐的工厂化生产．

Establishment of the Highly Frequency Regeneration System of Tetraploid Black Locust and Its Mericlone Production

A protocol is presented for direct and indirect regeneration of Tetraploid Black Locust from leaf explants. Orthogonal design method was adopted to make a study of effects of plant growth regulators on callus induction and differentiation of leaf explants. The selected optimal medium for callus induction was MS medium containing 0. 5 mg/L 6-BA and 1.0 mg/L NAA. The optimal medium for callus differentiation was MS medium supplemented with 2.0 mg/L 6-BA and 0. 2 mg/L NAA. The optimal medium for direct differentiation of leaves was MS medium supplemented with 2.0 mg/L 6-BA and 0. 1 mg/L NAA. The suitable formula for shoot rhizogenesis is 1/2 MS supplemented 0. 2 mg/L IBA, 0.1 mg/L NAA and 2 g/L AC with a rooting rate 100%. The survival rate of transplanting seedings is 90 %.