日落黄分子印迹电化学传感器的研制与应用

利用分子印迹技术,以日落黄为模板分子、邻氨基酚为功能单体,采用循环伏安法在石墨电极表面电聚合形成邻氨基酚聚合膜,经电化学方法在0.5mol/L H2SO4溶液中将模板分子去除,制得具有特异识别空穴的日落黄分子印迹修饰电极,然后,采用紫外可见分光光度法和电化学法对聚合薄膜进行表征.实验表明,该分子印迹修饰电极对日落黄有较高的结合速率、特异识别能力和灵敏度,-0.010V处微分脉冲伏安法的峰电流与日落黄的浓度在1.00×10-6～1.00×10-4 mol/L范围内呈良好的线性关系,且检出限为2.00×10-7 mol/L.运用该方法测定了饮料中的日落黄,回收率为95%～108%,为饮料中日落黄的选择性分析提供新的实验方法.

Preparation of sunset yellow sensor based on molecularly imprinting

A molecular imprinted film of sunset yellow with specific identification hole has been developed by molecularly imprinting technology.The sensitive film was prepared by cyclic voltammetry method on graphite electrode using o-aminophenol as functional monomer,sunset yellow as template which can be removed by electrochemical method in 0.5 mol/L H2SO4 solution.The polymer films were investigated by Uv-vis absorption spectrometry and electrochemical method.The results showed that the s had a sensitive response,a specific recognition and high selectivity towards the target sunset yellow.The linear relation between peak current of differential pulse voltammetry and concentration of sunset yellow were good in the range from 1.00×10-6 to 1.00×10-4 mol/L with a detection limit of 2.00×10-7 mol/L at potential of-0.010 V.The imprinted electrode has been applied to determinate sunset yellow in drink samples with recovery ranging from 95% to 108%.The molecular imprinted film can be exploited for the separative analysis of sunset yellow.