| 硫堇/聚2,6-吡啶二甲醛膜电极检测DNA及其损伤 |
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| 引用本文： | 赵常志,刘权,徐华筠,于莹.硫堇/聚2,6-吡啶二甲醛膜电极检测DNA及其损伤[J].辽宁师范大学学报(自然科学版),2010,33(1). |
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| 作者姓名： | 赵常志 刘权 徐华筠 于莹 |
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| 作者单位： | 生态化工教育部重点实验室,青岛科技大学,化学与分子工程学院,山东,青岛,266042 |
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| 基金项目： | 电分析化学国家重点实验室开放基金项目(0604) |
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| 摘 要： | 在玻碳电极上电聚合2,6-吡啶二甲醛,再共价键合硫堇至聚2,6-吡啶二甲醛膜的表面上制备成修饰电极.用电化学交流阻抗表征了该修饰电极的制备过程.以差示脉冲伏安法研究了该修饰电极对鲱鱼精DNA的作用,发现该修饰电极对dsDNA具有选择性的伏安响应.在优化的实验条件下,修饰电极氧化峰电流与dsDNA浓度的对数在0.10～100mg/L范围内呈现良好的线性关系,检测限为0.06mg/L.将该修饰电极用于检测鲱鱼精DNA的化学损伤,其峰电流与DNA的损伤程度成正比.该修饰电极制备简单、检测快速,5支电极间相对标准偏差小于4.83%.
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| 关 键 词： | 2 6-吡啶二甲醛 鲱鱼精DNA 硫堇 |
Electrochemical detecting of DNA and its damage based on thionine/Poly(pyridine-2,6-dicarbaldehyde) modified electrode |
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| ZHAO Chang-zhi,LIU Quan,XU Hua-jun,YU Ying.Electrochemical detecting of DNA and its damage based on thionine/Poly(pyridine-2,6-dicarbaldehyde) modified electrode[J].Journal of Liaoning Normal University(Natural Science Edition),2010,33(1). |
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| Authors: | ZHAO Chang-zhi LIU Quan XU Hua-jun YU Ying |
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| Institution: | Key Laboratory of Eco-chemical Engineering/a>;Ministry of Education/a>;College of Chemistry & Molecular Engineering/a>;Qingdao University of Science & Technology/a>;Qingdao 266042/a>;China |
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| Abstract: | A thionine/Poly(pyridine-2,6-dicarbaldehyde)modified electrode(Th/PPDA/GCE)was fabricated for detecting DNA and its damage.Th/PPDA/GCE was prepared by electropolymerizing pyridine-2,6-dicarbaldehyde on a glassy carbon electrode,and covalently immobilizing thionine on the surface of poly(pyridine-2,6-dicarbaldehyde)film.The preparation process and electrochemical responses of Th/PPDA/GCE were investigated by using electrochemical impedance spectroscopy and differential pulse voltammetry for herring sperm DNA... |
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| Keywords: | Polypyridine-2 6-dicarbaldehyde DNA damage thionine |
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