| 金鱼视觉系统内中、高分子量神经丝免疫组织化学定位 |
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| 引用本文： | WANG Fang-chun,王芳春,刘忠虎,司克媛,俞诗源.金鱼视觉系统内中、高分子量神经丝免疫组织化学定位[J].西北师范大学学报,2006,42(3):74-77. |
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| 作者姓名： | WANG Fang-chun 王芳春 刘忠虎 司克媛 俞诗源 |
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| 作者单位： | 西北师范大学生命科学学院，兰州大学生命科学学院，西北师范大学生命科学学院，西北师范大学生命科学学院 甘肃兰州730070，甘肃兰州730000，河南农业大学牧医工程学院，河南郑州450002，甘肃兰州730070，兰州大学生命科学学院，甘肃兰州730000，甘肃兰州730070 |
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| 基金项目： | 中国科学院资助项目;国家自然科学基金 |
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| 摘 要： | RT97是一种特异性识别中、高分子量神经丝蛋白抗原簇的抗体.采用组织化学技术对金鱼(Carassiusauratus)视觉系统内RT97识别神经丝的表达分布进行了观察.结果表明,RT97在金鱼视网膜、视神经和视顶盖中均有阳性反应:①在视网膜内视纤维层、内网层、内核层的水平细胞、光感受器的外节有RT97阳性反应;②在视乳突部和视神经有少量生长纤维为RT97阳性;③在视顶盖中RT97阳性反应集中在顶盖的边缘层(SM),同时观察到有RT97阳性纤维从中央纵突区(TL)通过顶盖腹面延伸至顶盖边缘区.实验结果提示,RT97不仅可作为金鱼视觉系统视觉生长纤维的一种标记蛋白,同时在视顶盖由RT97识别的蛋白也可能是一种吸引视觉纤维生长的靶蛋白.
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| 关 键 词： | 视网膜 视神经 视顶盖 免疫组化 |
| 文章编号： | 1001-988X（2006）03-0074-04 |
| 收稿时间： | 2005-11-02 |
| 修稿时间： | 2006-04-10 |
Immunohistochemical localization of the medium and high-weight neurofilament in the visual nervous system of Carassius auratus |
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| WANG Fang-chun.Immunohistochemical localization of the medium and high-weight neurofilament in the visual nervous system of Carassius auratus[J].Journal of Northwest Normal University Natural Science (Bimonthly),2006,42(3):74-77. |
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| Authors: | WANG Fang-chun |
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| Institution: | 1. College of Life Science, Northwest Normal University, Lanzhou 730070, Gansu, China;2. College of Life Science, Lanzhou University, Lanzhou 730000, Ganusu, China;3. College of Animal Husbandry and Veterinary, Henan Agriculture University, Zhengzhou 450002, Henan, China |
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| Abstract: | RT97 is known to be specific for high and medium-weight neurofilament subunits in mammals.RT-97 immunoreactivity in the visual nervous system of goldfish is studied by the immunochemical method.Our results demonstrate that the positive RT-97 immunoreactivity can be observed in the OFL,IPL,the horizontal cell of INL and the outer segment of the photoreceptive cells,a few of growing fibers can be observed to be positive in the optic nerve head and optic nerve.The RT-97 immunoreactivity mainly accumulate in the SM.And the positive RT-97 fibers can be observed to originate from the TL and go through the dorsal layers of the tectum to the SM.Our results indicate that RT-97 can be one good marker to illustrate the growing fibers and also one target candidate molecular in the tectal target to attract the retina fibers. |
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| Keywords: | RT97 |
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