| 菊花中总黄酮的提取工艺比较 |
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| 引用本文： | 刘小锐,申伟,梅林.菊花中总黄酮的提取工艺比较[J].西南师范大学学报(自然科学版),2011,36(6). |
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| 作者姓名： | 刘小锐 申伟 梅林 |
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| 作者单位： | 西南大学化学化工学院;中国人民解放军后勤工程学院油料应用于管理工程系; |
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| 摘 要： | 为优化超声波法和酶解法提取菊花总黄酮的工艺,并比较其工艺条件,选择因素水平,采用L9(34)正交设计优化提取工艺,以芦丁为对照品,用紫外分光光度计分析其提取率.结果表明,酶解法最佳工艺条件为:25倍70%的乙醇溶液,酶添加量0.5%,于45℃水浴下酶解150min,得率为5.60%;超声波法最佳工艺条件为:10倍70%的乙醇溶液超声40min,提取3次,得率为4.61%.酶解法的得率比超声波法的高21.5%.
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| 关 键 词： | 菊花 总黄酮 酶解法 超声法 提取 正交设计 |
@The Comparation of the Extraction Methods for Total Flavonoids of the Chrysanthemum |
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| LIU Xiao-rui,SHEN Wei,MEI Lin .School of Chemistry , Chemical Engineering,Southwest University,Chongqing,China,.Logistical Engineering Unversity of PLA,Shapingba Chongqing.@The Comparation of the Extraction Methods for Total Flavonoids of the Chrysanthemum[J].Journal of Southwest China Normal University(Natural Science),2011,36(6). |
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| Authors: | LIU Xiao-rui SHEN Wei MEI Lin School of Chemistry Chemical Engineering Southwest University Chongqing China Logistical Engineering Unversity of PLA Shapingba Chongqing |
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| Institution: | LIU Xiao-rui1,SHEN Wei1,MEI Lin2 1.School of Chemistry and Chemical Engineering,Southwest University,Chongqing,400715,China,2.Logistical Engineering Unversity of PLA,Shapingba Chongqing,401311 |
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| Abstract: | Objective To optimize the Ultrasonic Extraction and Enzymatic Hydrolysis Extraction,and to compare these two methods.Methods With the Rutin as the reference substance and ultraviolet spectrophotometer for analyzing the extraction rate,the extraction process is optimized after the level of factor is chosen and an orthogonal designing method L9(34) is adopted.Results The superior process conditions for the Enzymatic Hydrolysis Extraction are: 25 Ethanol solution(70%),with cellulose(0.5%),constant temperature(... |
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| Keywords: | Total Flavonoids Enzymatic Hydrolysis Ultrasound Extraction Extraction Orthogonal design |
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