| 家蚕碱性磷酸酶的功能基团研究 |
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| 引用本文： | 许敏,罗英,黄毅,鲁成,唐云明.家蚕碱性磷酸酶的功能基团研究[J].西南师范大学学报(自然科学版),2005,30(5):925-929. |
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| 作者姓名： | 许敏 罗英 黄毅 鲁成 唐云明 |
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| 作者单位： | 1. 西南大学,生命科学学院,重庆,400715
2. 西南大学,蚕桑学农业部重点实验室,重庆,400716 |
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| 基金项目： | 重庆市科委资助项目（2003-7852） |
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| 摘 要： | 用PCMB、NBS、PMSF、TNBS、SUAN、DTT、BrAc、IAc等在一定条件下选择性修饰家蚕碱性磷酸酶的部分氨基酸残基,对酶活力的变化进行测定.结果表明:PMSF、SUAN、BrAc、IAc和TNBS的修饰能显著抑制酶的活性,且酶活力降低程度与修饰剂的浓度相关;PMSF、DTT和NBT的修饰对酶的影响较小.初步认为,巯基和组氨酸的咪唑基可能是家蚕碱性磷酸酶的必需功能基团,赖氨酸残基和二硫键对保护酶的催化能力是必需的.
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| 关 键 词： | 碱性磷酸酶 功能基团 化学修饰 家蚕 |
| 文章编号： | 1000-5471（2005）05-0925-05 |
| 收稿时间： | 2004-12-02 |
| 修稿时间： | 2004年12月2日 |
Studies on the Functional Groups of Alkaline Phosphatase from Bombyx mori |
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| XU Min,LUO Ying,HUANG Yi,LU Cheng,TANG Yun-ming.Studies on the Functional Groups of Alkaline Phosphatase from Bombyx mori[J].Journal of Southwest China Normal University(Natural Science),2005,30(5):925-929. |
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| Authors: | XU Min LUO Ying HUANG Yi LU Cheng TANG Yun-ming |
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| Abstract: | The alkaline phosphatase from Bombyx mori were selectively modified by PCMB, NBS, PMSF, TNBS, SUAN, DTT, BrAc and IAc respectively, after that the changes in their activities have been detected. It was found that the reaction of AKP with PMSF, SUAN, BrAc, Iac and TNBS resulted in a strong inhibition of enzyme activities which decreased with the increase of modifier concentration. The activity of AKP with PMSF, DTT and NBS have been less inhibition. Studies of inactivation of AKP by chemical modification demonstrated that Cys-SH and His-imidazolyl are essential functional groups of the AKP. Partial disulfide bond and Lys residue are essential for the function of this enzyme. |
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| Keywords: | alkaline phosphatase functional groups~ chemical modification Bornbyx mori |
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