不同产地独活及其混伪品的质量差异性分析

多点采样,对不同来源的独活及其混伪品进行质量差异性分析.测定不同产地独活及其混伪品总灰分、酸不溶性灰分、蛇床子素和二氢欧山芹醇当归酸酯质量分数,并结合HPLC指纹图谱对不同产地独活及其混伪品开展质量评价.结果表明,不同产地独活除陕西样品的蛇床子素略低于《中华人民共和国药典》(以下简称《药典》)标准外,其余样品均符合《药典》质量要求,而独活混伪品的蛇床子素质量分数均远低于正品独活质量分数.不同产地的独活HPLC指纹图谱有25个共有峰,各独活样品的指纹图谱与对照图谱的相似度在0.9以上,所建立的指纹图谱可用于独活的质量评价.因此,多指标的测定结果结合HPLC指纹图谱,可有效对不同产地的独活质量进行差异性分析和评价;混伪品蛇床子素的质量分数远低于正品独活,蛇床子素的质量分数高低可作为正品独活区别于混伪品的一个重要特征指标.

On Quality Diversity Analysis of Angelica Pubescens f. Biserrata in Different Habitats and its Adulterants

Using multi-sampling method,different analysison sources of Angelica in different habitats and its adulterants was conducted.Determining the content of the totalash, acid-insoluble ash, ostholethe, columbianadin and the HPLC fingerprint in Angelica pubeseeas from different habitats and its adulterants, a research on the quality evaluation has been made. Results show that the contents of the samples examined complies with the requirement of the pharmacopoeia,except for Shanxi samples that the content of the ostholethe was slightly lower than the Pharmacopoeia. And the contents of the ostholethe of Angelica adulterants were considerably lower than Angelica.The HPLC fingerprinting was established from the samples, the data showed 25 characteristic peaks and the similarity of the fingerprints is greater than 0.9 in Angelica from different habitats for use as index peaks for qualitative identification. It is concluded that, combined with the HPLC fingerprint and the determination results of several indexes, we can get the difference analysis and evaluation of Angelica from different habitats. Content of the osthol in adulterants were considerably lower than certified A. pubescens f. biserrata, it was able to distinguish effectively the fake and quality goods.