| 狂犬病毒核蛋白在昆虫细胞中的表达及免疫原性的研究 |
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| 引用本文： | 于翔翔,谭有将,姚文荣,张永振.狂犬病毒核蛋白在昆虫细胞中的表达及免疫原性的研究[J].西南师范大学学报(自然科学版),2009,34(5). |
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| 作者姓名： | 于翔翔 谭有将 姚文荣 张永振 |
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| 作者单位： | 1. 石河子大学,生命科学学院,新疆,石河子,832003;中国疾病预防控制中心,传染病研究所,北京,102206
2. 中国疾病预防控制中心,传染病研究所,北京,102206 |
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| 摘 要： | 目的在昆虫细胞中表达出狂犬病毒核蛋白(NP),并探讨重组NP的免疫原性.方法采用杆状病毒表达系统在Sf9昆虫细胞中表达狂犬病毒核蛋白,用直接免疫荧光(DFA)、SDS-PAGE和Western blot对表达产物进行检测和分析,以感染重组杆状病毒的昆虫细胞裂解液免疫小鼠,检测表达产物的免疫原性.结果重组杆状病毒在昆虫细胞中获得表达,免疫小鼠可产生抗核蛋白抗体.结论在Sf9昆虫细胞中表达出狂犬病毒核蛋白,重组产物具有生物活性.
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| 关 键 词： | 狂犬病毒核蛋白 杆状病毒表达系统 免疫原性 |
Study on Expression and Immunogenicity of Rabies Virus Nucleoprotein |
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| YU Xiang-xiang,TAN You-jiang,YAO Wen-rong,ZHANG Yong-zhen.Study on Expression and Immunogenicity of Rabies Virus Nucleoprotein[J].Journal of Southwest China Normal University(Natural Science),2009,34(5). |
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| Authors: | YU Xiang-xiang TAN You-jiang YAO Wen-rong ZHANG Yong-zhen |
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| Abstract: | Objective: Study expression and immunogenicity of rabies virus nucleoprotein. Methods: Express rabies virus nucleoprotein in insect cells by using baculovirus expression system. The expressed nucleoprotein obtained from the cell culture was detected by direct immunofluorescence assay (DFA), SDS-PAGE and Western blot. Mice was immunized with the insect cell lysate. Result: The rabies virus nucleo-protein was expressed in insect cell. Mice produced anti-nucleoprotein antibody. Conclusion: The rabies virus nucleoprotein expressed in insect cell is biologically active. |
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| Keywords: | rabies virus nucleoprotein baculovirus expression system immunogenicity |
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