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| 流式细胞术检测活性污泥中微生物的群落结构 | |
| 引用本文： | 赵现方,陈林海,李宗义,王振河.流式细胞术检测活性污泥中微生物的群落结构[J].河南师范大学学报(自然科学版),2006,34(3):124-127. |
| 作者姓名： | 赵现方 陈林海 李宗义 王振河 |
| 作者单位： | 1. 河南科技学院,生命科技学院,河南,新乡,453003 2. 郑州大学,离子束生物工程实验室,郑州,450053 3. 河南师范大学,生命科学学院,河南,新乡,453007 |
| 摘 要： | 为了进一步了解活性污泥菌群结构及其功能,为污水处理的正常运转提供理论参考,本实验采用荧光原位杂交技术和流式细胞术对活性污泥细胞悬浊液进行微生物细胞检测分析,结果发现污泥中α-,β-和γ-变形杆菌亚纲和丛毛单胞菌属的微生物相对含量分别为15.09%,20.50%,12.00%和8.37%;这表明β-变形杆菌亚纲的含量最高,为活性污泥中的优势菌群. |
| 关 键 词： | 流式细胞术 活性污泥 荧光原位杂交 |
| 文章编号： | 1000-2367(2006)03-0124-04 |
| 收稿时间： | 2006-02-22 |
| 修稿时间： | 2006年2月22日 |
Flow Cytometric Analysis of Microbial Communities in Activated Sludge |
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| ZHAO Xian-fang,CHEN Lin-hai,LI Zong-yi,WANG Zhen-he.Flow Cytometric Analysis of Microbial Communities in Activated Sludge[J].Journal of Henan Normal University(Natural Science),2006,34(3):124-127. | |
| Authors: | ZHAO Xian-fang CHEN Lin-hai LI Zong-yi WANG Zhen-he |
| Institution: | 1.Life Science and Technology College, Henan Institute of Science and Technology,Xinxiang 453003,China; 2. Ion Beam Bioengineering Provincial Key Lab, Zhengzhou University,Zhengzhou 450053,China; 3. College of Life Science, Henan Normal University,Xinxiang 453007,China |
| Abstract: | In order to know in detail the microbial community and its function in activated sludge,and to offer reference in theory for wastewater treatment plants,this paper takes advantage of fluorescent insitu hybridization and Flow Cytometry to assay the microbial community structure in the sample.The results show that relative contents of α,β and γ subclass of Proteobacteria and Comamonas spp.are 15.09%,20.50%,12.00% and 8.37%,respectively,which suggests that βProteobacteria is the dominant microbial community in activated sludge. |
| Keywords: | flow cytometry activated sludge fluorescent in-situ hybridization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |