枯草芽孢杆菌XF-1的碳水化合物活性酶类(CAZymes)蛋白预测与遗传关系分析

枯草芽孢杆菌XF-1对多种植物病原菌具有较好的抑制作用,是农业生产上非常重要的生防菌之一.碳水化合物活性酶类蛋白(CAZymes)作为植物病原真菌、细菌中重要的蛋白,在其生长和发育过程中发挥着重要作用.基于前期获得的枯草芽孢杆菌XF-1中104个分泌蛋白基本序列信息,利用CAT预测程序对该菌中的CAZymes蛋白进行预测分析,明确其含有CAZymes蛋白的数量是58个,上述蛋白可以分为主要类别和复合类别两大类,前者包括26个GHs、21个CBMs以及2个CEs、3个PLs、2个GTs;后者则包括4个复合蛋白类型GH/CBM.该研究有效地实现了枯草芽孢杆菌XF-1中CAZymes的预测,为进一步开展其功能研究打下坚实的理论基础.

Prediction for CAZymes proteins fromBacillussubtilis XF-1 genome

Bacillus subtilis XF-1 has inhibitory effects on a variety of plant pathogens,which is an important bio-control agents on agricultural production.Carbohydrate active enzymes protein(CAZymes)was a large class of important proteins of plant pathogenic fungi and bacteria,which plays an important role in the growth and development process.Based on 104 secreted proteins obtained preliminary data,CAZymes protein was predicted with CAT forecasting procedures.And 58 CAZymes proteins were found in B.subtilis XF-1,which divided into major categories and composite category,and the former includes 26 GH,21 CBM,15 AA,2 CE,2 PL and 2 GT;the latter includes 4 GH/CBM.Through the above bioinformatics analysis,this research lay a solid theoretical foundation for further study its function.