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| 大鼠心肌肥厚中PTEN,p-PI_3K蛋白表达对心肌纤维化的影响 | |
| 引用本文： | 穆灵敏,王文锋,郭志坤,张光谋.大鼠心肌肥厚中PTEN,p-PI_3K蛋白表达对心肌纤维化的影响[J].河南师范大学学报(自然科学版),2012,40(3):128-130. |
| 作者姓名： | 穆灵敏 王文锋 郭志坤 张光谋 |
| 作者单位： | 1. 新乡医学院形态学实验室,河南新乡,453003 2. 新乡医学院生命科学技术系,河南新乡,453003 3. 新乡医学院河南省高等学校组织再生重点开放实验室,河南新乡,453003 |
| 摘 要： | 对健康成年SD大鼠采用皮下注射异丙肾上腺素造成心肌肥厚模型;取心肌组织,常规石蜡切片,Masson染色,观察心肌组织的成纤维细胞变化;免疫荧光染色检测PTEN和p-PI3K的表达及分布.结果与对照组相比,实验组细胞间纤维细胞增多;免疫荧光检测PTEN和p-PI3K的阳性表达明显增高.表明PTEN和p-PI3K蛋白表达增高可能在心肌纤维化的发生和发展过程中起重要作用. |
| 关 键 词： | PTEN p-PI3K 免疫组织化学 免疫荧光 心肌纤维化 |
Influence of PI3K and PTEN's Expression in Rats' Myocardial Hypertrophy for Myocardial Fibrosis |
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| MU Ling-min , WANG Wen-feng , GUO Zhi-kun , ZHANG Guang-mou.Influence of PI3K and PTEN's Expression in Rats' Myocardial Hypertrophy for Myocardial Fibrosis[J].Journal of Henan Normal University(Natural Science),2012,40(3):128-130. | |
| Authors: | MU Ling-min WANG Wen-feng GUO Zhi-kun ZHANG Guang-mou |
| Institution: | b(a.Morphologial Laboratory;b.Life Science and Technique Department;c.The Key Laboratory for Tissue Regeneration of Henan Universities,Xinxiang Medical College,Xinxiang 453003,China) |
| Abstract: | The research provides morphological data for the investigation of the cardiac hypertrophy signal transduction mechanism and reversal of cardiac hypertrophy by researching the expression of PTEN and p-PI3K in cardiac tissue in isoproterenol-induced myocardial hypertrophy.By researching the expression of PTEN and p-PI3K in cardiac tissue in isoproterenol-induced myocardial hypertrophy,the morphological data are provided for the investigation of the cardiac hypertrophy signal transduction mechanism and reversal of cardiac hypertrophy.Cardiac hypertrophy models are established by subcutaneous injection of isoproterenol on Healthy adult SD rats.The pathological changes of myocardial tissues with conventional paraffin section and HE staining are observed.The expression and distribution of PTEN and p-PI3K is detected by immunohistochemistry and immunofluorescence staining.Image analysis software is used to conduct quantitative analysis.Compared with the control group,the positive expression of PTEN and p-PI3K in the experimental group are significantly increased.The increase of PTEN and p-PI3K proteins expression may play an important role in the occurrence and development of cardiac hypertrophy. |
| Keywords: | PTEN p-PI3K immunohistochemistry immunofluorescence cardiachypertroph |
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