突变型P53和肿瘤易感基因101在肺癌组织中的表达及意义

探讨了P53基因突变对肺癌组织中TSG101基因的影响,结果是:TSG101在正常组织中呈强阳性表达100%(30/30),在高分化肺癌(包括肺鳞癌和肺腺癌)组织、低分化肺癌组织、淋巴结转移组织的表达分别为77.97%(92/118)、25.37%(17/67)、18.95%(18/95);P53的表达则相反,在正常组织、高分化肺癌组织、低分化肺癌组织、淋巴结转移组织的表达分别为6.67%(2/30)、67.80%(80/118)、86.57%(58/67)、94.63%(90/95);P53基因PCR-SSCP分析谱检测P53第5~8外显子阴性56例占30.27%,阳性129例占69.73%,总突变率为69.73%(127/180);P53与TSG101呈现负相关.这些结果提示P53和TSG101可能共同参与了肺癌的发生、发展和转移.

Expressions of Mutant P53 and Tumor Susceptibility Gene 101 in Lung Cancer

To investigate the effect of P53 gene mutation affecting on TSG101 gene in lung cancer tissues.Resuls:TSG101expression was strongly positive in normal tissue,expression rate of 100%(30/30);In high differentiated lung cancer(including lung squamous cell carcinoma and adenocarcinoma of the lung tissue),low differentiated lung cancer tissue,lymph node expression of metastasis tissues were 77.97%(92/118),25.37%(17/67),18.95%(18/95).On the contrary,the expression of P53 is different.Expression in normal tissue,differentiated lung cancer tissue,low differentiated lung cancer tissue,metastatic lymph node tissues were 6.67%(2/30),67.80%(80/118),86.57%(58/67),94.63%(90/95).P53 gene PCR-SSCP analysis spectya revealed that P53 exons 5-8 were negative in 56cases(30.27%)and positive in 129cases(69.73%).The total mutation rate was 69.73%(127/180).P53 is negatively correlated with TSG101.These results suggest that P53 and TSG101could be participated in the occurrence,development and metastasis of lung cancer