| 怀地黄HPLC指纹图谱研究 |
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| 引用本文： | 李建军,王君,王莹,陈雪娇,徐玉隔,张重义,江道会,赵正伟.怀地黄HPLC指纹图谱研究[J].河南师范大学学报(自然科学版),2014(3):119-124. |
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| 作者姓名： | 李建军 王君 王莹 陈雪娇 徐玉隔 张重义 江道会 赵正伟 |
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| 作者单位： | 河南师范大学生命科学学院;河南省高校道地中药材保育及利用工程技术研究中心;河南农业大学中药材研究所;宛西制药怀药生产基地;温县农业科学研究所; |
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| 基金项目： | 国家“十一五”科技支撑计划项目(2006BRI06R12-06);河南省教育厅自然科学研究计划项目(2009B180012);河南省重点科技攻关项目(122102310283);省教育厅科学技术研究重点项目(12B180010) |
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| 摘 要： | 目的:建立并比较怀地黄不同栽培品种HPLC指纹图谱,为强化怀地黄质量控制和品种鉴定与划分建立图谱标准.方法:采用HPLC,以0.4%醋酸溶液和乙腈进行梯度洗脱,检测波长为260nm;柱温:30℃;流速:1mL/min.使用"中药色谱指纹图谱相似度评价系统"进行数据处理,对不同栽培品种怀地黄的HPLC指纹图谱进行了相似度和聚类分析.结果:获得了HPLC优化技术条件;建立了含有18个特征指纹峰的怀地黄HPLC化学对照指纹图谱;9个农家品种划分为2大类,又可细分为4类.结论:该图谱可作为怀地黄质量控制的标准图谱,为怀地黄的质量控制、品种鉴别及划分提供理论和技术支撑.
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| 关 键 词： | 怀地黄 栽培品种 高效液相 指纹图谱 |
HPLC Chemical Fingerprint Chromatogram of Rehmannin Gtutinosa |
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| LI Jianjun;WANG Jun;WANG Ying;CHEN Xuejiao;XU Yuge;ZHANG Zhongyi;JIANG Daohui;ZHAO Zhengwei.HPLC Chemical Fingerprint Chromatogram of Rehmannin Gtutinosa[J].Journal of Henan Normal University(Natural Science),2014(3):119-124. |
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| Authors: | LI Jianjun;WANG Jun;WANG Ying;CHEN Xuejiao;XU Yuge;ZHANG Zhongyi;JIANG Daohui;ZHAO Zhengwei |
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| Institution: | LI Jianjun;WANG Jun;WANG Ying;CHEN Xuejiao;XU Yuge;ZHANG Zhongyi;JIANG Daohui;ZHAO Zhengwei;Henan Normal University College of Life Sciences,Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs,University of Henan Province;Institute of Chinese Medicinal Materials,Henan Agriculture University;The Wanxi Production Base of Pharmaceutical Huaiyao;The Agriculture Science Institute of Wenxian; |
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| Abstract: | Objective:To study the HPLC fingerprint of Rehmannin gtutinosa and establish a chromatographic standard for the quality control as well as to comparative study the HPLC fingerprint among various farm cultivars.Methods:The HPLC method was used.The mobile phase is 0.3%acetic acid and methyl cyanide,The wavelength is 260nm.The temprature is 30℃,and the flow rate is 1mL/min.The chromatographic fingerprint similarity evaluation system was adopted for data analysis.Results:Obtaining the HPLC optimization technology conditions and containing 20characteristic fingerprint peaks Rehmannia HPLC chemical control fingerprint;9farm species is divided into two categories,subdivided into four categories.The standard fingerprint chromatogram for Henan trueborn Rehmannin gtutinosa which contain 20characteristic peaks was built. Conclusion:The HPLC fingerprint method summaried in this article can be used as the typical chromatography and provide the theoretical and technical support for species identification and division of Rehmannin gtutinosa. |
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| Keywords: | Rehmannin gtutinosa farm cultivars high performance liquid chromatograph fingerprint chromtogram |
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