| 温度和光照对拟南芥VHA-c基因启动子活性的调节 |
| |
| 引用本文： | 于秀敏,武燕,王瑞刚.温度和光照对拟南芥VHA-c基因启动子活性的调节[J].河南师范大学学报(自然科学版),2013,41(4):120-123. |
| |
| 作者姓名： | 于秀敏 武燕 王瑞刚 |
| |
| 作者单位： | 内蒙古农业大学生命科学学院,呼和浩特,010018 |
| |
| 摘 要： | 转VHA-c2-GUS,VHA-c3-GUS和VHA-c5-GUS基因烟草在4℃和40℃温度胁迫以及25℃光/暗处理条件下叶片GUS表达水平表明,VHA-c具有光调控性,并可被逆境温度所调节.VHA-c2,VHA-c3和VHA-c5均可被4℃低温和光照促进表达.40℃高温可显著促进VHA-c3的表达,对VHA-c5有轻微的促进,而对VHA-c2则表现出轻微的抑制作用.
|
| 关 键 词： | 拟南芥 VHA-c GUS 温度 光照 |
Temperature and Light Regulates the Promoter Activity of VHA-c Genes of Arabidopsis Thaliana |
| |
| YU Xiumin
,
WU Yan
,
WANG Ruigang.Temperature and Light Regulates the Promoter Activity of VHA-c Genes of Arabidopsis Thaliana[J].Journal of Henan Normal University(Natural Science),2013,41(4):120-123. |
| |
| Authors: | YU Xiumin WU Yan WANG Ruigang |
| |
| Institution: | (College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China) |
| |
| Abstract: | To analyze the promoter activity of VHA-c genes of Arabidopsis thaliana,The GUS repoter gene was fused with the promoter of VHA-c2,c3and c5respectively,and the VHA-c-GUSconstructs were transformed into tobacco leaves.Expression analysis of GUS activity under 4℃,40℃ or 25℃showed that all the three promoters were induced by low temperature,while the VHA-c3promoter was up-regulated under 40 ℃,but the VHA-c2promoter was inhibited slightly under the same conditions.Light also up-regulated GUS expression driven by the three promoters respectively,indicating that all the three promoters were responsive to light. |
| |
| Keywords: | Arabidopsis thaliana VHA-c GUS temperature light |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
