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| 斑马鱼SAA基因克隆及表达分析 | |
| 引用本文： | 齐麟,向志明,张彤.斑马鱼SAA基因克隆及表达分析[J].河南师范大学学报(自然科学版),2011,39(5):134-136. |
| 作者姓名： | 齐麟 向志明 张彤 |
| 作者单位： | 1. 铁道警官高等专科学校治安系,郑州 450003;中山大学生命科学学院,广州 510275 2. 中国科学院南海海洋研究所,广州,510300 3. 河南大学生命科学学院,河南开封,475000 |
| 基金项目： | 有害生物控制与资源利用国家重点实验室开放课题(SKLBC2010K06); 公安部科技创新项目(2010YYCXTDGZ125) |
| 摘 要： | 利用巢式PCR(nest PCR)的方法克隆斑马鱼血清淀粉样蛋白A基因,并进行生物信息学分析.实时荧光定量PCR结果显示:SAA在斑马鱼胚胎发育时期的表达量呈现明显的“增长—稳定—增长”趋势.成鱼组织分布中,SAA在11个斑马鱼组织中均能检测到,在心脏和肝脏中相对高表达. |
| 关 键 词： | 斑马鱼 血清淀粉样蛋白A 实时荧光定量PCR |
Cloning and Expressing Analysis of SAA in Zebrafish |
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| QI Lin,XIANG Zhi-ming,ZHANG Tong.Cloning and Expressing Analysis of SAA in Zebrafish[J].Journal of Henan Normal University(Natural Science),2011,39(5):134-136. | |
| Authors: | QI Lin XIANG Zhi-ming ZHANG Tong |
| Institution: | QI Lin1,2,XIANG Zhi-ming3,ZHANG Tong4(1.Department of Public Security,Railway Police College,Zhengzhou 450003,China,2.College of Life Sciences,Sun Yat-sen University,Guangzhou 510275,3.South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510300,4.College of Life Sciences,Henan University,Kaifeng 475004,China) |
| Abstract: | A full length ORF of serum amyloid A(SAA) is isolated using nest PCR method in zebrafish.The ORF region encoding 121 amino acid residues is 366bp in length.The deduced mass weight is 13.62kDa and the PI is 9.65.The realtime qPCR result indicates that SAA mRNA level is in increasing-stabilization-increasing pattern during the development period.SAA is expressed in the 11 zebrafish tissues,and highly expressed in heart and liver. |
| Keywords: | zebrafish SAA realtime qPCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |