| 发酵液中透明质酸含量测定方法的比较研究 |
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| 引用本文： | 刘屹然,陈林海,李宗伟,王雁萍,杨天佑,谈重芳,和朝军,秦广雍,霍裕平.发酵液中透明质酸含量测定方法的比较研究[J].河南师范大学学报(自然科学版),2006,34(1):102-105. |
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| 作者姓名： | 刘屹然 陈林海 李宗伟 王雁萍 杨天佑 谈重芳 和朝军 秦广雍 霍裕平 |
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| 作者单位： | 郑州大学,离子束生物工程省重点实验室,郑州,450052 |
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| 摘 要： | 比较了Bitter-Muir法和CTAB浊度法用于测定透明质酸质量浓度的准确度和精确度,发现在没有杂质的情况下,二者都适用于透明质酸浓度的测定.研究了葡萄糖、蛋白质、无机盐和酵母膏等发酵液成分对2种测量方法的影响,结果表明,这些物质对Bitter-Muir法存在较大影响,而对CTAB浊度法的干扰较小.CTAB浊度法的操作简便快捷,发酵液无需预处理即可直接测定.
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| 关 键 词： | 透明质酸 发酵液 定量分析 Bitter-Muir法 CTAB浊度法 |
| 文章编号： | 1000-2367(2006)01-0102-04 |
| 收稿时间： | 2005-07-28 |
| 修稿时间： | 2005年7月28日 |
Comparative Study on Two Quantitative Assays of Hyaluronic Acid in Fermentation Broth |
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| LIU Yi-ran,CHEN Lin-hai,LI Zong-wei,WANG Yan-ping,YANG Tian-you,TAN Zhong-fang,HE Zhao-jun,QIN Guang-yong,HUO Yu-ping.Comparative Study on Two Quantitative Assays of Hyaluronic Acid in Fermentation Broth[J].Journal of Henan Normal University(Natural Science),2006,34(1):102-105. |
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| Authors: | LIU Yi-ran CHEN Lin-hai LI Zong-wei WANG Yan-ping YANG Tian-you TAN Zhong-fang HE Zhao-jun QIN Guang-yong HUO Yu-ping |
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| Institution: | Henan Province Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China |
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| Abstract: | The accuracy and precision of Bitter-Muir method and CTAB turbidimetric method were examined to assay the concentration of hyaluronic acid in fermentation broth.The result shows that both methods work successfuly when impurities don't exist.The affects of constituents in fermentation broth, such as glucose,protein,inorganic salt and yeast extract,were investigated,and the result shows that the presence of these constituents has a considerable interference for Bitter-Muir method,but only slight interference for CTAB method.Owing to its simplicity and rapidity,CTAB method can be directly used to analyze HA fermentation broth without pretreatment. |
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| Keywords: | hyaluronic acid fermentation broth quantitative analysis Bitter-Muir method CTAB turbidimetrie method |
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