| 产辅酶Q10热带假丝酵母菌株C-1发酵过程优化 |
| |
| 引用本文： | 石若夫,熊璐璐.产辅酶Q10热带假丝酵母菌株C-1发酵过程优化[J].南京理工大学学报(自然科学版),2008,32(5). |
| |
| 作者姓名： | 石若夫 熊璐璐 |
| |
| 作者单位： | 1. 南京理工大学,化工学院,江苏,南京,210094
2. 盐城工学院,评估处,江苏,盐城,224003 |
| |
| 摘 要： | 该文以热带假丝酵母（candida tropicalis）C-1为CoQ10的生产菌，通过发酵过程的优化，重点考察了碳源、氮源、接种量、酸碱度和装液量等对生物量和CoQ10产量的影响。发现氮源对产物合成影响最大，其次分别是酸碱度、装液量和碳源。优化后的发酵条件是：碳源，葡萄糖26g／L＋蔗糖26g／L；氮源，酵母膏7．5g／L＋蛋白胨7．5g／L；pH为6．5，装液量为40mL，发酵时间为30h，培养温度为30℃，CoQ10的得率为10．8mg／L。此外，加入适量的维生素B1、烟酸和铁离子等有利于CoQ10微生物细胞的生长和CoQ10产量的提高。
|
| 关 键 词： | 产辅酶Q10 热带假丝酵母 发酵条件 |
Optimization of Fermentation Process of CoQ10 Produced by Candida Tropicalis (Strain C-1) |
| |
| SHI Ruo-fu,XIONG Lu-lu.Optimization of Fermentation Process of CoQ10 Produced by Candida Tropicalis (Strain C-1)[J].Journal of Nanjing University of Science and Technology(Nature Science),2008,32(5). |
| |
| Authors: | SHI Ruo-fu XIONG Lu-lu |
| |
| Institution: | 1.School of Chemical Engineering;NUST;Nanjing 210094;China;2.Department of Education Evaluation;Yancheng Institute of Technology;Yancheng 224003;China |
| |
| Abstract: | The fermentation process of candida tropicalis(strain C-1) is optimized.The effect of some ferment conditions,including carbonaceous,nitrogenaceous,inoculating volume,pH and medium volume,on the biomass and content of CoQ10 are researched.The orthogonal test is used to analyze these fermentation conditions.The results show that nitrogenaceous is very important to CoQ10 synthesis and that pH,medium volume,and carbonaceous are in the next place.An elementary ferment condition is gained,that is,carbonaceous(gl... |
| |
| Keywords: | CoQ10 candida tropicalis fermentation conditions |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
