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| 重组鲑鱼降钙素融合蛋白工程菌发酵工艺研究 | |
| 引用本文： | 付海云,王红权,何建勇.重组鲑鱼降钙素融合蛋白工程菌发酵工艺研究[J].河北科技大学学报,2006,27(1):54-56,69. |
| 作者姓名： | 付海云 王红权 何建勇 |
| 作者单位： | 1. 沈阳药科大学制药工程学院,辽宁,沈阳,110016;石家庄制药集团有限公司,河北,石家庄,050051 2. 石家庄制药集团有限公司,河北,石家庄,050051 3. 沈阳药科大学制药工程学院,辽宁,沈阳,110016 |
| 摘 要： | 为研究大肠杆菌表达重组鲑鱼降钙素融合蛋白(GST-sCT-Gly)工程菌的发酵工艺,采用瑞士比欧生物工程公司40 L发酵罐发酵,对影响工程菌生长和目的蛋白的表达条件进行优化;在优化条件下,以40 L发酵罐发酵工程菌,菌体收获量可达到57 g/L,目的蛋白表达量占总蛋白的23.5%;结果表明,研究确定的发酵工艺可以提高工程菌收获量及目的蛋白的表达量,且稳定可靠,可应用于生产。 |
| 关 键 词： | 降钙素 重组 大肠杆菌 发酵 |
| 文章编号： | 1008-1542(2006)01-0054-03 |
| 收稿时间： | 2005/6/14 0:00:00 |
| 修稿时间： | 2005年6月14日 |
Study on fermentation process of fusion protein of recombinantsalmon calcitonin expressing E.coli |
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| FU Hai-yun,WANG Hong-quan and HE Jian-yong.Study on fermentation process of fusion protein of recombinantsalmon calcitonin expressing E.coli[J].Journal of Hebei University of Science and Technology,2006,27(1):54-56,69. | |
| Authors: | FU Hai-yun WANG Hong-quan and HE Jian-yong |
| Institution: | College of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang Liaoning 110016,China;Shijiazhuang Pharmaceutical Group Ltd., Shijiazhuang Hebei 050051, China;College of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang Liaoning 110016,China |
| Abstract: | Objective:To develop the best fermentation process on E.coli expressing fusion protein of recombinant salmon calcitonin GST-sCT-Gly).Methods:The expression pf recombinant fusion protein was analyzed to optimize the composition of medium,induction time and other factors in the process of fermentation.Results:Under the estabilished condition,57 g of wet bacteria per liter was obtained and the derivative of recombinant fusion protein was about 23.5% of total protein on host.Conclusion:The established fermentative technique increases the collection efficiency and expression of recombinant fusion protein.The fermentation process was steady,and able to be used in pilot production. |
| Keywords: | calcitonin recombinant E coli fermentation |
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