食品中叶酸分析方法及稳定性研究进展

通过优化样品前处理体系,建立精确的叶酸分析检测方法,对促进叶酸的多方面、深层次研究具有迫切的现实意义。介绍了叶酸的分子结构及其主要存在形式,以及常见食品中叶酸的含量。阐述了化学法和酶解法等样品前处理方法,分析表明在测定富含淀粉、蛋白质的豆类、谷物等食品中的叶酸时,更适合采用与淀粉酶、蛋白酶等联合使用的酶解法。综述了微生物法、荧光分析法、液相色谱法等常见叶酸检测方法,指出高效液相色谱-质谱联用法可快速测定不同形式的叶酸,且具有高效、精确等特点。探讨了影响叶酸稳定性的因素,以及提高叶酸摄入量和稳定性的措施,认为可通过在主粮作物中添加叶酸、蛋白质胶囊包裹叶酸、促进叶酸与蛋白质结合等方式来提高叶酸的摄入量及其稳定性。

Advances in research on analysis methods and stability of folic acid in food

It is urgently and practically significant to optimize the sample pretreatment, and to establish accurate detection of folic acid content in food and the presence of folic acid, in order to promote the in-depth research of various aspects of folic acid. In the paper, the molecular structure of folic acid and its main forms, as well as the content of folic acid in common foods are introduced. The sample pretreatment methods such as chemical method and enzymatic hydrolysis method are described. It is indicated that the enzymatic hydrolysis method is more suitable for the determination of folic acid in foods such as beans and grains, so that the folic acid combined with protein, starch and the like is fully hydrolyzed by using a combination of protease and amylase. The common methods for detecting folic acid, such as microbial method, fluorescence analysis method and high performance liquid chromatography (HPLC), are reviewed. HPLC is characterized by high efficiency and precision, especially ultra-performance liquid chromatography-mass spectrometry/-mass spectrometry (UPLC-MS/MS), which can quickly determine different forms of folic acid. Factors affecting the stability of folic acid and measures to increase folic acid intake and stability are explored.It shows that the measures to increase folic acid intake and stability includes adding folic acid in staple crops, packaging folic acid with protein capsule, facilitating the combination of folic acid and protein, etc.