黑色素瘤模型的建立与评价

为了快速有效地进行在体药物筛选，研究了以小鼠黑色素实体瘤组织中的原代细胞构建黑色素瘤模型的方法。从荷黑色素瘤小鼠的组织中提取原代肿瘤细胞，与体外培养的B16细胞通过皮下注射分别植入两组C57BL/6小鼠体内，考察两组小鼠肿瘤显现时间和生长速度。结果表明：当接种浓度为5.0×10⁶个/ mL、每只0.2 mL时，小鼠黑色素瘤原代细胞和体外培养的B16细胞成瘤率分别为100%和80%，且小鼠右前肢肿瘤(大小约4 mm³）出现时间分别约在第3天和第8天。紫杉醇对两种模型的初步评价显示，与体外培养的B16细胞模型相比，原代B16细胞模型是体内筛选和评价特定化合物抗肿瘤活性的一种可行而有效的方法，成瘤时间较短、成瘤率高，所得实验数据误差也较小。模型的建立为相关药物评价模型的开发研究提供了一条可借鉴的新途径。

Establishment and evaluation of melanoma model

In order to screen drug rapidly and effectively in vivo, the approach of the establishment for mouse melanoma model with primary cell from melanin solid tumor tissue of mouse is studied. The primary cell and the cultured B16 cells in vitro are implanted into two groups of C57BL/6 mice by hypodermic, respectively. The appearing time and the growth rate of tumor in two groups of mouse are investigated. The results show that when the inoculation is 0.2 mL for each one (the cell concentration of 5.0×10⁶/mL), the tumor formation rates of the primary tumor cells of mouse melanoma and in vitro cultured B16 cells are 100% and 80%, respectively, and it takes about 3 and 8 days respectively that the tumor sizes become about 4 mm³. The preliminary evaluation of two models are carried out with taxol, showing that the primary B16 cell model is a feasible and effective method for screening and evaluating the antitumor activity of specific compounds in vivo compared with the B16 cell model cultured in vitro, and it has shorter tumor forming time, higher tumor forming rate and less error in the obtained experimental data, which provides a new way for the development and research of related drug evaluation models.