东北铁线莲总多酚的超声提取工艺研究

探索超声波辅助提取东北铁线莲总多酚的最佳工艺条件。以东北铁线莲总多酚的提取率作为评价指标,选择乙醇体积分数、提取时间、料液比、提取次数为考察因素,采用正交试验法确定了东北铁线莲中总多酚的最佳工艺,采用分光光度法对提取液中总多酚的含量进行了测定,检测波长为766nm。结果表明:最佳提取工艺条件中乙醇浓度为55%(体积分数),提取时间为35min,料液比(质量比)为1︰30,提取次数为3次。没食子酸在质量浓度为1.116～11.160μg/mL时呈现良好的线性关系。该提取方法操作简单,结果比较可靠,适用于东北铁线莲总多酚的提取。

Study on extraction technology of total polyphenols in Clematis Manshurica Rupr.

This paper is to establish the optimum extraction technology of total polyphenols(TP) in Clematis manshurica Rupr.by ultrasonic extraction method.By using the extraction efficiency of TP in Clematis manshurica Rupr.as evaluating indicator,some important factors,such as the concentration of ethanol,the extracting time,the ratio between raw material and solvent and the extracting times,are optimized by orthogonal experimental design.The content of TP in Clematis manshurica Rupr.is determined by spectrum photometric method at detection wavelength of 766 nm.The optimum extraction condition of TP in Clematis manshurica Rupr.with ultrasonic method are obtained as follows: the concentration of ethanol,the extracting time is 35 min,the ratio of raw material to solvent is 1∶30,and the extraction times is 3 times is 55%.The concentration of gallic acid and its absorption value shows a good linear relationship at the range of 1.116~11.160 μg/mL.It is concluded that the ultrasonic extraction method obtained in the paper is easy to operate and reliable,so it could be used for extracting TP in Clematis manshurica Rupr.