| 地衣芽孢杆菌海水生化需氧量传感器研究 |
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| 引用本文： | 马莉,崔建升,王晓辉,刘俊稚,张洁.地衣芽孢杆菌海水生化需氧量传感器研究[J].河北科技大学学报,2004,25(4):83-85. |
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| 作者姓名： | 马莉 崔建升 王晓辉 刘俊稚 张洁 |
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| 作者单位： | 河北科技大学环境科学与工程学院,河北石家庄,050018 |
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| 基金项目： | 国家"八六三"计划课题(2001AA635100) |
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| 摘 要： | 用经海水驯化的地衣芽孢杆菌作响应菌株,采用夹层膜法制作固定化微生物膜,以氧电极为换能器制成BOD生物传感器;以空白海水为底液,传感器在BOD质量浓度为0～27mg/L范围内线性相关系数r=0.99978,对BOD质量浓度为4.31mg/L的GGA溶液连续平行测定7次,相对标准偏差为2.56%。
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| 关 键 词： | 生化需氧量 微生物生物传感器 地衣芽孢杆菌 海水 |
| 文章编号： | 1008-1542(2004)04-0083-03 |
| 修稿时间： | 2004年6月29日 |
BOD Biosensor for Seawater Using Bacillus Licheni Formis |
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| MA Li,CUI Jian-sheng,WANG Xiao-hui,LIU Jun-zhi,ZHANG Jie.BOD Biosensor for Seawater Using Bacillus Licheni Formis[J].Journal of Hebei University of Science and Technology,2004,25(4):83-85. |
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| Authors: | MA Li CUI Jian-sheng WANG Xiao-hui LIU Jun-zhi ZHANG Jie |
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| Abstract: | Based on oxygen electrode,a BOD biosensor for seawater has been fabricated with immobilized cells using double-layered membrane method. The biological sensing material is immobilized Bacillus licheniformis tamed in saline water. With blank seawater as substrate, characteristics of the biosensor are tested. The biosensor has a linear response of 0 to 27 mg/L BOD and the linearity is perfect(r=0.999 78). The standard deviation of continuous measurements (n=7) is 2.56%. The sensor shows stability within 40 days without any decrease in signal. |
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| Keywords: | BOD microbial biosensor bacillus licheni formis seawater |
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