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| 木鳖子皂苷提取分离工艺研究 | |
| 引用本文： | 姜仁辉,林慧彬,林建强.木鳖子皂苷提取分离工艺研究[J].山东师范大学学报(自然科学版),2009,24(1):82-84. |
| 作者姓名： | 姜仁辉 林慧彬 林建强 |
| 作者单位： | 1. 山东大学微生物技术国家重点实验室,250100,济南;山东省济南卫生学校,250023,济南 2. 山东省中医药研究院,250014,济南 3. 山东大学微生物技术国家重点实验室,250100,济南 |
| 基金项目： | 国家科技部科技支撑项目 |
| 摘 要： | 皂苷为木鳖子主要药效成分之一．本文对木鳖子皂苷的几种提取方法和分离纯化方法进行比较，以期得到一种能够快速简便的提取和纯化方法．考察了木鳖子皂苷的浸渍提取、超声提取、闪式提取对提取率的影响，并进行比较；把小鳖子皂苷的粗提物，分别采用正丁醇萃取和大孔吸附树脂法进行纯化．结果显示，超声提取的提取率最高；闪式提取时间最短；经大孔树脂处理纯度提高，为54．17％；超声或闪式提取结合大孔树脂纯化，成本低、操作简便易行、具有实用性． |
| 关 键 词： | 木鳖子 皂苷 分离 闪式提取 大孔树脂 |
STUDIES ON THE EXTRACTION AND PURIFICATION PROCESS OF Momordica cochinchinensis spreng |
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| Jiang Renhui,Lin Huibin,Lin Jianqiang.STUDIES ON THE EXTRACTION AND PURIFICATION PROCESS OF Momordica cochinchinensis spreng[J].Journal of Shandong Normal University(Natural Science),2009,24(1):82-84. | |
| Authors: | Jiang Renhui Lin Huibin Lin Jianqiang |
| Institution: | Jiang Renhui, Lin Huibin, Lin Jianqiang ( 1 State Key Laboratory of Microbial Technology, Shandong Uniersity, 250100, Jinan, China; 2 Jinan Medical School, 250023, Jinan, China; 3Shandong Research Institute of Chinese Traditional Medicine,250014,Jinan, China ) |
| Abstract: | Saponins are the major effective components in Momordica cochinchinensis Spreng. The extraction and purification methods of the saponin were studied in order to obtain a quick and effective extraction and purification method. The methods of dipping extraction, ultrasonic and flash - extraction were used, and the extraction efficiencies of different methods were compared. The crude extract saponins were purified by macroporous resin and n - butanol alcohol extract, respectively. The results showed that ultraviolet method was of highest extraction efficiency, and flash - extraction consumed shortest time. The macroporous resin separation method obtained the highest purity of 54.17%. The ultraviolet of flash - extraction combined with macroporous resin purification was proved to be the low cost and effective method in real applications. |
| Keywords: | Momordica cochinchinensis Spreng saponin seperation flash - extraction macmporous resin |
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