| 人造沸石及浸提剂对植物脯氨酸含量测定的影响 |
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| 引用本文： | 杜希华,侯福林,杨培林.人造沸石及浸提剂对植物脯氨酸含量测定的影响[J].山东师范大学学报(自然科学版),2000,15(3):301-303. |
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| 作者姓名： | 杜希华 侯福林 杨培林 |
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| 作者单位： | 1. 山东师范大学生物学系,250014,济南
2. 山东师范大学测试中心,250014,济南 |
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| 摘 要： | 采用测定植物叶片中游离脯氨酸含量的改进乙醇法和磺基水杨酸法，比较分析了人造沸石和浸提剂测定结果的影响。结果表明：人造沸石吸附作用明显，普遍降低脯氨酸回收率，使样品测定值下降；磺基水杨酸做浸提剂样品回收率高，方法简便、准确可靠。
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| 关 键 词： | 植物 叶片 游离脯氨酸 人造沸石 浸提剂 测定 |
EFFECTS OF PERMUTET AND EXTRACTANTS ON THE CONTENT DETERMINATION OF FREE PROLINE IN PLANT LEAVES |
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| Du Xihua,Hou Fulin,Yang Peilin.EFFECTS OF PERMUTET AND EXTRACTANTS ON THE CONTENT DETERMINATION OF FREE PROLINE IN PLANT LEAVES[J].Journal of Shandong Normal University(Natural Science),2000,15(3):301-303. |
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| Authors: | Du Xihua Hou Fulin Yang Peilin |
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| Institution: | Du Xihua
,Hou Fulin
;(Department of Biology,Shandong Normal University,250014,Jinan,P R C);Yang Peilin
;(Testing Centre,Shandong Normal University,250014,Jinan,P R C) |
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| Abstract: | The effects of permutet and extractants on the content determination of free Proline in plant leaves were studied.The results showed that Proline was distinctly absorbed by permutet,and as results,the recovery rates of Proline were lower and the contents determined in plant leaves reduced oberviously.The method using sulfosalicylic acid as extractant was better than that using alcohol because of its higher Proline recovery rate,uncomplicated steps,and higher accuracy. |
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| Keywords: | plant leaves free Proline permutet extractants |
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