| 参芪颗粒中黄芪甲苷含量的测定 |
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| 引用本文： | 陈雷,黄帧桧.参芪颗粒中黄芪甲苷含量的测定[J].安徽理工大学学报(自然科学版),2012(2):67-69. |
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| 作者姓名： | 陈雷 黄帧桧 |
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| 作者单位： | 皖南医学院药理教研室;淮南职业技术学院药理教研室 |
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| 基金项目： | 淮南职业技术学院科学研究资助项目(HKJ11-4) |
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| 摘 要： | 目的:建立高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定黄芪甲苷含量的方法。方法:采用蒸发光散射检测器(ELSD)以黄芪甲苷为对照品对参芪颗粒中黄芪甲苷进行HPLC分析,色谱柱Agilent C18(250 mm×4.6 mm,5μm),流动相乙腈-水(32:68),流速0.8mL/min,柱温30℃,ELSD漂移管温度105℃,载气流速2.5 L/min。结果:黄芪甲苷在30.48μg/mL～304.8μg/mL范围内线性关系良好,回收率为97.64%(,RSD=1.11%)。结论:HPLC-ELSD法具有良好的精密度和重现性,可用于参芪颗粒中黄芪甲苷含量测定的质量控制。
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| 关 键 词： | 高效液相色谱 蒸发光散射检测器 参芪颗粒 黄芪甲苷 |
Measurement of Astragaloside in Shenqi Granules |
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| CHEN Lei,HUANG Zheng-gui.Measurement of Astragaloside in Shenqi Granules[J].Journal of Anhui University of Science and Technology:Natural Science,2012(2):67-69. |
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| Authors: | CHEN Lei HUANG Zheng-gui |
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| Institution: | 1(1.Department of Pharmacology,Wannan Medical College,Wuhu Anhui 241002,China;2.Department of Pharmacology,Huainan Vocational Technical College,Huainan Anhui 232001,China) |
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| Abstract: | The research is to establish the method using the high-performance liquid chromatography-evaporative light scattering detection(HPLC-ELSD) to determine the contents of Astragaloside.ELSD was used to measure the contents of Astragaloside in Shenqi granules on the basis of astragaloside standards for control with HPLC analysis.Other conditions were set as follows: Chromatographic column Agilent C18(250 mm×4.6 mm,5 μm),mobile phase of acetonitrile-water(32:68),flow rate 0.8 in mL/min,column temperature at 30 ℃,ELSD drift tube temperature at 105 ℃,flow rate of carrier gas in 2.5 L/min.The Results showed that when astragaloside is between 30.48 μg/mL and 304.8 μg/mL,the linear relationship is fine and the recovery rate is 97.64%(RSD = 1.11%).HPLC-ELSD is precise and reproducible,and can be used to control the quality of the preparation. |
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| Keywords: | High performance liquid chromatography evaporative light scattering detector Shenqi granules astragaloside |
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