| 小鼠LEAP-2成熟肽基因在毕赤酵母中的分泌表达 |
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| 引用本文： | 王红亮.小鼠LEAP-2成熟肽基因在毕赤酵母中的分泌表达[J].新乡学院学报(自然科学版),2011(4):333-335. |
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| 作者姓名： | 王红亮 |
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| 作者单位： | 新乡学院数学系 |
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| 摘 要： | 实验利用已构建的分泌型重组表达质粒pPIC9-mmLEAP-2，氯化锂转化表达宿主菌GS115毕赤酵母；应用表型和PCR技术筛选高拷贝转化株。经摇瓶发酵和甲醇诱导，SDS—PAGE分析重组mmLEAP-2表达量，为进一步研究mmLEAP-2的活性奠定了基础。
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| 关 键 词： | 小鼠肝表达抗菌肽-2成熟肽 毕赤酵母 分泌表达 |
Secretion Expression of Mature Mouse Liver-expressed Antimicrobial Peptide-2 in Pichia Pastoris |
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| Authors: | WANG Hong-liang |
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| Institution: | WANG Hong-liang(Department of Mathematics,Xinxiang University,Xinxiang 453003,China) |
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| Abstract: | The pPIC9-mmLEAP-2 expression vector was transformed into Pichia pastoris GS 115, the transform with multi-copy inserts were selected by PCR identification, the expression of recombinant mmLEAP-2 induced by methanol was analyzed by SDS-PAGE, which provides a basis for further research on antibacterial activity of mmLEAP-2. |
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| Keywords: | mmLEAP-2 Pichia pastoris secretion expression |
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