| 固氮鱼腥藻(Anabaena azotica Ley HB686)氮固定的能量和还原剂来源 |
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| 引用本文： | 陈兆平.固氮鱼腥藻(Anabaena azotica Ley HB686)氮固定的能量和还原剂来源[J].华南师范大学学报(自然科学版),1990(2). |
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| 作者姓名： | 陈兆平 |
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| 作者单位： | 华南师范大学生物系 |
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| 基金项目： | 国家自然科学基金资助课题 |
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| 摘 要： | 某些外源碳水化合物提高固氮鱼腥藻的固氮能力.在光照和5%氧浓度条件下,分子氢促进固氮作用.光合抑制剂、呼吸链抑制剂和解偶联剂均抑制固氮酶活性,若同时加入葡萄糖,DCMU的抑制作用被解除,而对DBMIB和解偶联剂的作用影响甚微.固氮鱼腥藻固氮作用的还原剂与光合作用产生的碳化合物有关,能量主要由循环光合磷酸化供给.
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| 关 键 词： | 固氮鱼腥藻 固氮作用 固氮酶活性 还原剂 循环光合磷酸化 |
SOURCES OF ENERGY AND REDUCTANT FOR NITROGEN FIXATION IN ANABAENA AZOTICA LEY HB686 |
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| Chen Zhaoping.SOURCES OF ENERGY AND REDUCTANT FOR NITROGEN FIXATION IN ANABAENA AZOTICA LEY HB686[J].Journal of South China Normal University(Natural Science Edition),1990(2). |
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| Authors: | Chen Zhaoping |
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| Institution: | Biol. Dept. |
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| Abstract: | Several exogenous carbohydrates were found to stimulate C2H2 reduction activity in A. azotica HB686. The stimulation in light was more distinct than in dark. H2-supported C2H2-reduction was strictly light-dependent and low O2concentration ( 5 % ) was indispensable. C2H2-reduction was suppressed by photosynthetic chain inhibitors. The inhibition with DCMU was lessened by addition of grucose, whereas with DBMIB was little affected. Electron transfer inhibitors for respiratory chain and uncoupler inhibited C2H2 reduction activity. The results showed that the reductant supporting nitrogenase activity ultimately came from carbohydrates generated by photosynthesis in the vegetative cell, and ATP for nitrogenase was mainly generated by cyclic photophosphorylation and was supplemented by oxidative phosphorylation in A, azotica HB686. |
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| Keywords: | Anabaena azotica Ley HB686 nitrogen fixation nitrogenase activity reductant cyclic photophosphorylation |
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