牡蛎寡肽对免疫低下小鼠模免疫功能的影响

研究牡蛎寡肽对免疫低下小鼠免疫功能的调节作用。采用腹腔注射环磷酰胺（CTX）建立免疫低下小鼠模型，研究不同剂量牡蛎寡肽对小鼠免疫器官脏器指数、脾淋巴细胞转化增殖情况、NK细胞活性、小鼠体液免疫、巨噬细胞吞噬能力、血清TNF-、IL-6和溶血素水平的影响。结果显示，与免疫抑制模型组小鼠相比，牡蛎寡肽能够显著提高脾淋巴细胞增殖能力、NK细胞活性、廓清指数K、吞噬指数a、吞噬中性红能力、TNF-、IL-6、溶血素水平和脾淋巴细胞CD3+4T淋巴细胞亚群及CD3+8T淋巴细胞亚群分布（P0.05），而对小鼠的肝、脾脏指数影响不显著。结果表明牡蛎寡肽能够提高由CTX引起的免疫低下模型小鼠的细胞免疫、体液免疫及非特异性免疫功能，对小鼠的免疫功能具有正面调控的作用。

Effect of Oyster Oligo-peptide on Immunologic Fucction in Immunosuppressive Mice

The effect of oyster oligopeptide on regulation of immune function in cyclophosphamide (CTX)-induced immunosuppressive mice was evaluated. Different dosages of oyster oligopeptide (50、100、200 mg/kg) were given to the low, middle, and high group mice. The group injected with CTX was adopted as model group, with the group without treatment as the control group. The index of immune organs such as liver, spleen and so on, the transformation and propagation ratio of T lymphocyte, the activity of NK cell, the humoral immunity, macrophage phagocytosis, the level of cell cytokines and hemolysin were all assessed. The experiment results revealed that compared with the model immunosuppressed group, oyster oligopeptide could significantly enhance the proliferation of spleen lymphocyte, NK cell activity, expurgation index k, devouring index a, ability of devouring neutral red, the level of TNF-、IL-6 and hemolysin, and regulate the distribution of CD3+4T and CD3+8T spleen lymphocyte subsets. However, the influence of oyster oligopeptide on the immune organs was not significant. Hence, it was concluded that oyster oligopeptide can improve the cell immunity, humoral immunity and non-specific immune function of immunosuppressive mice induced by CTX, and it can regulate the immune function positively in mice.