巨噬细胞增强宫颈癌细胞对SN-38的抗性

通过GDSC在线软件，分析了SN-38在数据库中8种宫颈癌细胞系对310多种化疗药的半抑制质量浓度(IC₅₀)Z分数值，并选取HPV-18阳性HeLa细胞、HPV-16阳性SiHa和CaSki细胞以及HPV阴性C-33A细胞，经过SN-38处理24、48 h，通过CCK8试验检测了细胞活力来探究巨噬细胞是否介导宫颈癌细胞对SN-38抵抗. THP1被HeLa和SiHa细胞诱导成肿瘤相关巨噬细胞，通过CCK8试验探讨巨噬细胞在宫颈癌细胞对SN-38敏感性中的作用. 结果显示:CaSki、ME-180、HT-3、C-33A对SN-38极敏感，HeLa和SiHa对SN-38敏感，而CAL-39对SN-38不敏感；CaSki和C-33A的IC₅₀值相对于HeLa和SiHa更低，表明CaSki和C-33A对SN-38更敏感；巨噬细胞显著抑制SN-38对HeLa和SiHa细胞的杀伤. 这些结果表明了肿瘤相关巨噬细胞促进宫颈癌细胞对SN-38抵抗.

Macrophage's Promotion of Cervical Cancer Cell Resistance to SN-38

GDSC (Genomics of Drug Sensitivity in Cancer) online database was analyzed and the IC₅₀ Z Score values of 8 cervical cancer cell lines under more than 310 chemotherapy drugs were obtained. Then HPV-18 positive HeLa cells, HPV-16 positive SiHa and CaSki cells and HPV negative C-33A cells were treated with SN-38 for 24 and 48 hours and the cell viability was detected with CCK8 experiment. Finally, THP1 cells were induced into tumor-associated macrophages (TAM) to explore the role of TAM in the sensitivity of cervical cancer cells to SN-38. The results showed that CaSki, ME-180, HT-3 and C-33A were very sensitive to SN-38, HeLa and SiHa were relatively sensitive to SN-38, and CAL-39 was not sensitive to SN-38. In addition, IC₅₀ values of CaSki and C-33A were lower than those of HeLa and SiHa, indicating that CaSki and C-33A were more sensitive to SN-38. TAM promoted HeLa and SiHa cell resistance to SN-38. In conclusion, the results suggested that TAM promoted the resistance of cervical cancer cells to SN-38.