| 黑米皮花色苷的大孔树脂吸附纯化研究 |
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| 引用本文： | 侯方丽,张名位,苏东晓,张瑞芬,魏振承,张雁,池建伟,唐小俊.黑米皮花色苷的大孔树脂吸附纯化研究[J].华南师范大学学报(自然科学版),2009,1(1):100. |
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| 作者姓名： | 侯方丽 张名位 苏东晓 张瑞芬 魏振承 张雁 池建伟 唐小俊 |
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| 作者单位： | 1.广东省农业科学院农业生物技术研究所 |
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| 基金项目： | 国家自然科学基金，国家科技支撑计划 |
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| 摘 要： | 摘要:比较了5种大孔吸附树脂对黑米皮花色苷（ Black Rice Anthocyanins， BRA）的吸附纯化效果，研究了AB-8型大孔树脂对BRA的吸附与解吸特性.结果表明:AB-8大孔树脂对BRA具有较好的吸附和解吸能力，是吸附纯化BRA的最佳树脂类型，其分离纯化BRA的最佳工艺参数为:上柱液pH 值=2，样品质量浓度1.0mg/mL，吸附流速1.0mL/min，以体积分数为70%乙醇为解吸剂，洗脱速度为1.0mL/min. 树脂的重复利用率好，使用5次后吸附率无显著性差异(P0.05)，使用7次后，吸附率仅降低2.58%. BRA经AB-8大孔树脂纯化后，花色苷含量提高2.38倍，总抗氧化能力提高3.99倍.
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| 关 键 词： | 黑米皮花色苷 AB-8大孔吸附树脂 吸附性能 |
| 收稿时间： | 2008-07-04 |
RESEARCH ON ADSORPTION CHARACTER AND PURIFICATION OF BLACK RICE ANTHOCYANINS WITH MACROPOROUS RESIN |
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| HOU Fang-li,ZHANG Ming-wei,SU Dong-xiao,ZHANG Rui-fen,WEI Zhen-cheng,ZHANG Yan,CHI Jian-wei,TANG Xiao-jun.RESEARCH ON ADSORPTION CHARACTER AND PURIFICATION OF BLACK RICE ANTHOCYANINS WITH MACROPOROUS RESIN[J].Journal of South China Normal University(Natural Science Edition),2009,1(1):100. |
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| Authors: | HOU Fang-li ZHANG Ming-wei SU Dong-xiao ZHANG Rui-fen WEI Zhen-cheng ZHANG Yan CHI Jian-wei TANG Xiao-jun |
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| Institution: | 1.College of Food Science & Technology;Huazhong Agricultural University;Wuhan 430070;China;2.Key Laboratory of Functional Food Research for Ministry of Agriculture;Guangdong Academy of Agricultural Sciences;Guangzhou 510610;China |
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| Abstract: | The absorption and purification effects of 5 kinds of resins on Black Rice Anthocyanins(BRA) were compared,and the properties of adsorption and desorption for AB-8 on BRA were further investigated.The result shows that AB-8 is the best resin for BRA due to its better adsorption and desorption capability.The optimal technical parameters of AB-8 are as follows: the pH of solution is 2.0;the anthocyanins concentration of the sample solution is 1.0 mg/mL,and the adsorption flow rate is 1mL /min;the desorption f... |
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| Keywords: | black rice anthocyanins AB-8 adsorption character |
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