| 嗜热脱硫菌BSM培养基成分响应面法优化 |
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| 引用本文： | 贺琳琳,游海鹏,祝加伟,郭凯,隋媛红,吕英海.嗜热脱硫菌BSM培养基成分响应面法优化[J].山东科技大学学报(自然科学版),2014(5):58-62. |
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| 作者姓名： | 贺琳琳 游海鹏 祝加伟 郭凯 隋媛红 吕英海 |
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| 作者单位： | 山东科技大学化学与环境工程学院; |
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| 基金项目： | 国家级大学生创新创业训练计划项目 |
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| 摘 要： | 采用单因素分析法、显著因子筛选实验设计法、爬坡实验设计法和响应面分析法,对534-16嗜热脱硫菌的培养基成分和培养条件进行了优化研究。结果表明,当培养基的主要成分为:葡萄糖21.00g/L,酵母粉5.25g/L,Na2HPO4·2H2O 6.60g/L时,脱硫菌生长速率最快。最适合菌种生长的条件为:培养基pH值为7.0,培养温度为53.39℃。
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| 关 键 词： | 嗜热脱硫菌 培养基 成分 优化 响应面法 |
Response Surface Methodology to Optimize BSM Medium Component of Thermophile Desulfurization Bacteria |
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| Institution: | He Linlin,You Haipeng, Zhu Jiawei, Guo Kai, Sui Yuanhong,Lu Yinghai (College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao,Shandong 266590, China) |
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| Abstract: | Single factor analysis, significant factor analysis, slope-climbing experiment and response surface method- ology were used to optimize the medium＇s component and condition of desulfurization strain of 534-16. The results indicate that the optimum ratio for the maximum of the growth rate is 21 g/L glucose, 5.25 g/L yeast powder and 6.60 g/L Naz HPO4 ·2H2 O; temperature and pH value are the two primary factors to influence the desulfurization strain＇s growth rate;the optimized temperature is 53.39 ℃ and optimized pH value is 7.0. |
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| Keywords: | thermophile desulfurization strain medium~ component optimization response surface methodology |
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