便携式癌细胞荧光显微成像系统的设计与实现

根据荧光成像在癌症诊断分析中的机理，选择高效率的光学系统，配合高精度的半数字式对焦技术和智能图像采集融合技术，实现高性能便携式癌细胞荧光成像系统。系统选择多波段的LED光源；采用科学级面阵互补金属氧化物半导体(CMOS)探测器，配合散斑抑制二向色镜，降低背景干扰，提高检测灵敏度；采用半数字式对焦技术，实现显微镜高精细的自动对焦；采用FPGA+万兆网的结构，实现图像数据的高速传输。实验表明，基于该系统可制成质量为4 kg、功耗不到20 W的便携式癌细胞荧光成像设备，实现清晰的多谱段癌细胞荧光成像和快速检测。

Design and Implementation of a Portable Fluorescence Microscopic Imaging System for Cancer Cells

Based on the mechanism of fluorescence imaging in cancer diagnosis and analysis,a high performance portable cancer cell fluorescence imaging system is developed with a high efficiency optical system,using high precision semi digital focusing technology and intelligent image acquisition and fusion technology.The system selects multi band LED light source,uses sci area array CMOS detector and speckle suppression dichroic mirror,which reduces background interference and improve detection sensitivity,adopts semi digital focusing technology which achieves high precision autofocus of the microscopeand applies sing FPGA+10 Gigabit network structure so that high speed transmission of image data is realized.Experiments show that based on this system,a portable fluorescence imaging device for cancer cells with a mass of 4 kg and a power consumption of less than 20 W can be made,which can realize clear multi spectral fluorescence imaging and rapid detection of cancer cells.