细胞色素C在汞表面生长性质的AFM研究——吸附自组装机理

利用原子力显微镜对细胞色素C在汞表面生长过程进行研究，发现细胞色素C在汞表面首先形成的是高度稍大于1nm的吸附单分子层，随着生长时间的增加，吸附在汞表面的细胞色素C分子和溶液中的分子问相互作用，自组装形成生长活性点，生长速度明显加快，生长时间达到8min时，细胞色素C能形成底部直径达527nm，高度达138nm，分布密度约为1．2～2．3个/μm^2的细胞色素C分子集聚体．生长机理是吸附自组装机理．细胞色素C的浓度较大地影响其在Hg表面的自组装平衡，随着浓度的减少，细胞色素C所能形成的最大分子集聚体的高度和数目都随之减少。浓度与平均表现高度是非线性的关系．

Studies on Growth Process of Cytochrome at Mercury Surface with Atomic Force Microscope:Absorbing and Self-assembling Process

The growth process of cytochrome at the mercury surface was studied with AFM. The single molecule of CC which was a little more than 1 nm high was absorbed at mercury surface at the first minute. The absorbing speed was accelerated when the molecules of CC were interacted at the mercury surface, and then they were further self-assembled to form active bodies. At the eighth minute, the aggregated bodies of CC were formed with the distribution of 1.2-2.3 per/μm^2 which were about 138nm in height and about 527nm in diameter. The content of CC solution had great effect on the self-assembling balance. With the decrease of content, the number and height of self-assembling bodies was reduced. The relationship between the content and average height was nonlinearizcd.