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| Au@SiO2修饰电极的制备及对Cyt c的直接电化学研究 | |
| 引用本文： | 周宇艳,鲜跃仲,刘芳,胡艺,周丽绘,金利通.Au@SiO2修饰电极的制备及对Cyt c的直接电化学研究[J].华东师范大学学报(自然科学版),2005,2005(3):48-52. |
| 作者姓名： | 周宇艳 鲜跃仲 刘芳 胡艺 周丽绘 金利通 |
| 作者单位： | 1. 华东师范大学,化学系,上海,200062;湖南大学,化学生物传感与计量学国家重点实验室,长沙,410082 2. 华东理工大学,分析测试中心,上海,200237 3. 华东师范大学,化学系,上海,200062 |
| 基金项目： | 国家自然科学基金(20305007);上海市启明星计划(04QM×1421);湖南大学化学生物传感与计量学国家重点实验室开放基金以及教育部博士点基金(20030269014). |
| 摘 要： | 研究Au@SiO2复合纳米材料修饰电极的制备方法及其对细胞色素c (Cyt c)的电催化行为.Cyt c在该修饰电极上表现出一对氧化还原峰，且在1.0×10-7~2.0×10-5 mol/L浓度范围内，Cyt c峰电流与浓度呈良好的线性关系，检测下限为5.0×10-8 mol/L (S/N=3).还应用紫外可见光谱考察了Au@SiO2复合纳米材料的生物兼容性，结果表明，Au@SiO2材料为Cyt c提供了一个“近水”的微环境，使其在该修饰电极表面不易变性，因而具有良好的生物相容性. |
| 关 键 词： | Cyt c Au@SiO2 复合纳米材料 Cyt c Au@SiO2 复合纳米材料 |
| 文章编号： | 1000-5641（2005）03-0048-05 |
| 收稿时间： | 2004-9-15 |
| 修稿时间： | 2004年9月1日 |
Preparation of the Au@SiO2 Modified Electrode and Its Application to the Direct Electrochemistry of Cytochrome C(Chinese) |
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| ZHOU Yu-yan,XIAN Yue-zhong,LIU Fang,HU Yi,ZHOU Li-hui,JIN Li-tong.Preparation of the Au@SiO2 Modified Electrode and Its Application to the Direct Electrochemistry of Cytochrome C(Chinese)[J].Journal of East China Normal University(Natural Science),2005,2005(3):48-52. | |
| Authors: | ZHOU Yu-yan XIAN Yue-zhong LIU Fang HU Yi ZHOU Li-hui JIN Li-tong |
| Institution: | 1.Department of Chemistry, East China Normal University, Shanghai200062,China;2.State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University,Changsha410082, China;3.Center of Analysis and Research, East China University of Science and Technology,Shanghai2000237,China |
| Abstract: | Au@SiO2 composite nanoparticles were prepared. By controlling synthesis conditions, the thickness of the silica shell could be varied in the range of tens to several hundred nanometers. Coupled with chitosan (CHIT), Au@SiO2/CHIT/GC modified electrode was fabricated. The electrode was applied to the determination of cytochrome c (Cyt c). The results of the experiments indicated that the currents of Cyt c were linear to its concentrations over the range from 1.0×10-7 to 2.0×10-5 mol/L and the calculated detection limits (S/N=3) was 5.0×10-8 mol/L. Further work could be done to apply this electrochemical method to preparing biosensor and detecting biologic samples. |
| Keywords: | cytochrome c Au@SiO2 nano composite material |
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