葛根黄酮降血糖防治糖尿病并发症的实验研究

通过尾静脉注射四氧嘧啶(50mg/kg体重)建立糖尿病小鼠模型,实验分为正常组、糖尿病组、糖脉康药物对照组和葛根黄酮组,实验40d后检测各组动物体重、饮水量、血糖、糖耐量、果糖胺、山梨醇、醛糖还原酶、山梨醇脱氢酶和糖基化终产物(AGEs)等实验指标.结果表明,葛根黄酮能明显改善糖尿病小鼠的病理症状,降低血糖水平,降低血液中果糖胺、山梨醇、醛糖还原酶和AGEs的含量,升高血液中山梨醇脱氢酶的含量,同时还能降低肝脏、肾脏、大脑、心脏和皮肤组织中AGEs和山梨醇的含量,与糖尿病组小鼠比较差异显著(P0.01).由此得出结论,葛根黄酮除具有明显降血糖作用外,还对糖尿病并发症有一定的预防和治疗效果.

Experimental study on the hypoglycemic action and prevention of diabetic complication with Pueraria flavonoids

An animal model of diabetes was established with alloxan tail vein injection (50 mg/kg), in order to research the effects of Pueraria flavonoids on blood glucose level and the prevention of diabetes and its complication.The mice were randomly divided into 4 groups: normal group, diabetic group, Tang Mai Kang control group and Pueraria flavonoids group.After 40 days, the weight, water intake, blood glucose, sugar tolerance, fructosamine, D-glucitol, aldose reductase, SDH and advanced glycation end products(AGEs) of the mice were detected.Compared with the diabetic group, the blood glucose level of the mice treated with Pueraria flavonoids was decreased markedly, while the content of SDH in the blood was increased significantly.The contents of fructosamine, D-glucitol, aldose reductase and AGEs in the blood were reduced obviously.Moreover, the contents of AGEs and D-glucitol in the liver, kidney, cerebrum, heart and skin were also decreased (all P＜0.01).Our results demonstrate that Pueraria flavonoids are effective not only in lowering blood glucose level but also in the prevention or treatment diabetes-induced complication.