孕妇尿液表皮生长因子的分离,纯化及活性检测

孕发尿液蛋白丙酮粉粗提液经Ｂｉｏ－ｇｅｌＰ－１０凝胶层析获得五个组分，经免疫点印渍分析后，证明其中组分Ⅲ的活性最高，该组分再经ＳｅｐｈａｄｅｘＧ－１５凝胶层析，又得到Ａ、Ｂ、Ｃ和Ｄ四个组，ｈＥＧＦ活性主要分布在组分Ａ中。本文建立了免疫点印渍方法并应用于ｈＥＦＧ的活性检测。

Isolation and Purification of hEGF from Pregnant's Urine

The study presented was about the preparation of hEGF from pregnant's urine. The isolation procedure involved two-step chromatography. In the first step, the main active fractionwas fraction ill of the five fractions from Bio-gel P-10 column chromatography, the active fraction went again on sephadex G-15 and four fractions was obtained, but the activity concentrated was on peak A. The molecular weight of hEGF sample was about 6, 000 identified byHPLC. Immuno-Dot blotting was applied to detect of hEGF activity and met with success. Themethod was simply, fast and sensitive. The hEGF sample had obvious activity to stimulate eyelids open and tooth eruption of new born mice.