| 大孔树脂纯化天麻多糖的工艺研究 |
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| 引用本文： | 陈琛,李鑫鑫,魏唯,刘祥,田慧玲,冯振斌,胡红忠,郑红星.大孔树脂纯化天麻多糖的工艺研究[J].四川大学学报(自然科学版),2018,55(5):1109-1115. |
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| 作者姓名： | 陈琛 李鑫鑫 魏唯 刘祥 田慧玲 冯振斌 胡红忠 郑红星 |
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| 作者单位： | 陕西理工大学生物科学与工程学院/陕西省天麻山茱萸工程技术研究中心/陕西理工大学中德天然产物研究所;陕西汉王药业有限公司;陕西百圣生物工程有限公司 |
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| 基金项目： | 国家高端外国专家项目（GDW20146100228，GDT20176100048）；国家重点引智项目（W20166100045）；陕西省科技统筹创新工程计划项目（2015KTTSSF01-03，2015HBGC-18）；陕西省重点研发计划项目（2016NY-180）；陕西省协同创新计划项目（2016XT-13）；陕南秦巴山区生物资源综合开发协同创新中心资助项目（QBXT-Z(P)-15-21） |
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| 摘 要： | 本研究以炮制的干天麻为原料,水提醇沉法提取多糖,大孔吸附树脂纯化,比较了八种大孔树脂(AB-8、D101、LX-17、D301、NKA-9、S-8、LSD-001、ADS-7)对天麻多糖静态吸附-解析效果,筛选出最佳纯化树脂,再研究最佳树脂纯化天麻多糖工艺参数.结果为:八种大孔吸附树脂中D101对天麻多糖的纯化效果最好.样品液浓度、温度、上样速度,洗脱用乙醇浓度、洗脱流速及洗脱体积等因素均对D101树脂吸附分离天麻多糖有影响.所得的最佳纯化工艺为:20℃是较适宜的吸附温度,上样速度1BV/h,上样浓度4mg/mL,进行吸附;吸附饱和平衡后,用解析液浓度60%乙醇,解析速率2BV/h,解析液体积3BV进行动态洗脱.通过该工艺天麻多糖的纯度提高到了65.7%,表明了大孔树脂D101对天麻多糖具有较好的纯化效果.
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| 关 键 词： | 多糖 大孔吸附树脂 吸附 纯化 天麻 |
| 收稿时间： | 2017/10/6 0:00:00 |
| 修稿时间： | 2018/1/2 0:00:00 |
Macroporous adsorption resin for the purification of polysaccharides from Gastrodia elata Bl |
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| CHEN Chen,LI Xin-Xin,WEI Wei,LIU Xiang,TIAN Hui-Ling,FENG Zhen-Bin,HU Hong-Zhong and ZHENG Hong-Xing.Macroporous adsorption resin for the purification of polysaccharides from Gastrodia elata Bl[J].Journal of Sichuan University (Natural Science Edition),2018,55(5):1109-1115. |
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| Authors: | CHEN Chen LI Xin-Xin WEI Wei LIU Xiang TIAN Hui-Ling FENG Zhen-Bin HU Hong-Zhong and ZHENG Hong-Xing |
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| Institution: | Chinese German Joint Institute for Natural Product Research/Shaanxi Engineering Research Center of Tall Gastrodia Tuber and Medical Dogwood/College of Biological Science and Engineering, Shaanxi University of Technology,Chinese German Joint Institute for Natural Product Research/Shaanxi Engineering Research Center of Tall Gastrodia Tuber and Medical Dogwood/College of Biological Science and Engineering, Shaanxi University of Technology,Chinese German Joint Institute for Natural Product Research/Shaanxi Engineering Research Center of Tall Gastrodia Tuber and Medical Dogwood/College of Biological Science and Engineering, Shaanxi University of Technology,Chinese German Joint Institute for Natural Product Research/Shaanxi Engineering Research Center of Tall Gastrodia Tuber and Medical Dogwood/College of Biological Science and Engineering, Shaanxi University of Technology,Shaanxi Hanwang Chinese Medicine Co.,Ltd,Shaanxi Hanwang Chinese Medicine Co.,Ltd,Shaanxi Baisheng Biological Engineering Co.,Ltd and Chinese German Joint Institute for Natural Product Research/Shaanxi Engineering Research Center of Tall Gastrodia Tuber and Medical Dogwood/College of Biological Science and Engineering, Shaanxi University of Technology |
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| Abstract: | |
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| Keywords: | Polysaccharide Macroporous resin Adsorption Purification Gastrodia elata |
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