两种麻疯树核糖体失活蛋白基因Curcin和Curcin C在种子萌发过程中的表达模式研究

为了研究和开发丰富的麻疯树资源,本研究以四川省西昌市金沙江干热河谷地区的麻疯树种子为材料,将种子浸水萌发后收集各组织用于实验。qRT-PCR结果显示胚乳中Curcin、Curcin C基因表达先增加后减少,在子叶中Curcin C基因的表达也是如此。Western Blot结果显示Curcin蛋白在根、下胚轴、真叶、子叶中不表达,Curcin C蛋白在根和下胚轴中也不表达,而在真叶、子叶中表达。生物信息学软件预测及亚细胞定位结果都显示Curcin:GFP和Curcin C:GFP融合蛋白定位在细胞壁上。本研究初步阐明了两种麻疯树核糖体失活蛋白Curcin、Curcin C的表达模式并对其进行了亚细胞定位。

Study on the expression patterns of two ribosome inactivated protein genes Curcin and Curcin C of Jatropha curcas during the process of seed germination

In order to research and develop the abundant resources of Jatropha curcas, the seeds of Jatropha curcas from the dry hot valley of Jinsha River in Xichang City, Sichuan Province were used as materials. The tissues for experiment were collected after soaking seeds in water to germinate. The qRT PCR results showed that the expression of Curcin and Curcin C genes in endosperm increased first and then decreased, as did the expression of Curcin C gene in cotyledons. Western Blot results showed that Curcin protein was not expressed in roots, hypocotyls, true leaves and cotyledons, Curcin C protein was also not expressed in roots and hypocotyls, but expressed in true leaves and cotyledons. Both bioinformatics software prediction and subcellular localization results showed that Curcin:GFP and CurcinC:GFP fusion proteins were localized in the cell wall. In this study, the expression patterns of two ribosome-inactivated proteins Curcin and Curcin C in Jatropha curcas were preliminarily elucidated and subcellular localization was performed.