| 产碱性脂肪酶酵母菌的分离与鉴定 |
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| 引用本文： | 严建华,蒋芳,刘成君.产碱性脂肪酶酵母菌的分离与鉴定[J].四川大学学报(自然科学版),2004,41(6):1280-1283. |
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| 作者姓名： | 严建华 蒋芳 刘成君 |
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| 作者单位： | 四川大学生命科学学院,成都610064 |
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| 摘 要： | 从成都龙泉果园采集的160份土壤样品中，通过观察油脂平板上水解圈直径与菌落直径的大小之比(D／d)，分离到12株产碱性脂肪酶的真菌，采用滴定法测液体培养物酶活进行复筛，获得一株产酶活性最高的酵母菌，通过对该酵母菌进行形态、生理生化特征的观察与测试，初步鉴定为胶淀粉假丝酵母，命名为Candida amylolenta L-30。
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| 关 键 词： | 胶淀粉假丝酵母菌 碱性脂肪酶 分离 鉴定 |
| 文章编号： | 0490-6756(2004)06-1280-04 |
The Isolation and Identification of an Alkaline Lipase-producing Yeast |
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| YAN Jian-hua,JIANG Fang,LIU Cheng-jun.The Isolation and Identification of an Alkaline Lipase-producing Yeast[J].Journal of Sichuan University (Natural Science Edition),2004,41(6):1280-1283. |
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| Authors: | YAN Jian-hua JIANG Fang LIU Cheng-jun |
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| Abstract: | Twelve alkaline lipase-producing epiphytes were isolated from the soil samples of longquan orchards in Chengdu, through observing diameter ratio between digested zone and colony(D/d) in plates.By rescreening, using titration to test the enzyme activity of these strains'liquid culturists, the highest activety yeast was obtainded and coded L-30. Utilizing the morphology, physiology and biochemistry methods,It was identified as Candida amylolenta. |
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| Keywords: | Candida amylolenta alkaline lipase isolation identification |
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