TPM：基于Taverna的PCR引物设计与评估工作流系统

PCR引物设计是PCR实验中关键而重要的一步，而引物的特异性情况直接影响着PCR实验的成败. 首先，设计了基于多因素（序列相似性，引物3′末端自由能，Tm等）的PCR引物特异性评估工具——MFEprimer；其次，在MFEprimer的基础上，结合引物设计软件primer3，在Taverna工作流平台上构建了PCR引物设计（primer3）与评估（MFEprimer）一体的工作流系统——TPM（Taverna-Primer3-MFEprimer），实现了从引物设计到评估的自动化分析流程，使用简单方便.

TPM: a Taverna workflow for PCR primer design and evaluation

Primer design is one of the important steps in PCR experiments and the specific primers are crucial for successful PCR reactions. Firstly, a program named MFEprimer was proposed to evaluate the specificity of PCR primers by considering sequence similarity, stability at the 3' end of the primer and melting temperature. Secondly, by combining MFEprimer and primer designing tool primer3, a Taverna workflow called TPM (Taverna-Primer3-MFEprimer) was developed to perform primer design and primer evaluation in one step. TPM is accurate, reliable and easy to use.